| Research Title |
A real-time PCR and melting curve analysis for rapid detection of Schistosoma mekongi DNA in infected snails and rat feces |
| Date of Distribution |
11 May 2012 |
| Conference |
| Title of the Conference |
The first Symposium of Specific problem in Greater Mekong Sub-region (SHep-GMS) |
| Organiser |
Health Cluster, The National Research University Project, Khon Kaen University, Thailand |
| Conference Place |
Auditorium 1, Faculty of Medicine, Khon Kaen University |
| Province/State |
Khon Kaen |
| Conference Date |
11 May 2012 |
| To |
11 May 2012 |
| Proceeding Paper |
| Volume |
1 |
| Issue |
1 |
| Page |
29 |
| Editors/edition/publisher |
Health Cluster, The National Research University Project, Khon Kaen University, Thailand |
| Abstract |
Schistosomiasis is a tropical parasitic disease caused by blood-dwelling fluke worms of the genus Schistosoma whose infective stages, the cercariae, are amplified through mollusks acting as intermediate hosts. People are infected when exposed to fresh water containing cercariae that penetrate the skin. There are however considerable differences in intensity of infection and morbidity, depending on the pattern of exposure and the infective species. In Asia, 3 Schistosoma species cause human infection: Schistosoma japonicum, Schistosoma malayensis, and Schistosoma mekongi. S. mekongi, a blood-dwelling fluke is a water-borne parasite that found in communities along the lower Mekong river basin i.e. Cambodia and Lao PDR. This study was developed a real-time PCR assay combined with melting curve analysis to detect S. mekongi in experimentally infected snails and in fecal samples of infected rats. The procedure is based on the melting curve analysis of a hybrid between an amplicon from S. mekongi mitochondrion sequence and specific fluorophore-labeled probes. The method gave high sensitivity and specificity, could be applies as a fast and reliable tool for cercarial location in environment water in endemic areas, for epidemiological studies and eradication programs for intermediate hosts and for diagnosis of human infection in Asian populations as well as travelers returning from high-risk areas. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
true |
| Award Title |
The Best Poster Presentation |
| Type of award |
รางวัลด้านวิชาการ วิชาชีพ |
| Organiser |
Health Cluster, The National Research University Project, Khon Kaen University, Thailand |
| Date of awarding |
11 พฤษภาคม 2555 |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
ไทย