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Publication
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Research Title |
The effects of dilution rate and packed-bed volume on continuous ethanol fermentation from sweet sorghum juice by immobilized yeasts on sweet sorghum stalk |
Date of Distribution |
29 August 2011 |
Conference |
Title of the Conference |
The 4th International Conference on Fermentation Technology for Value Added Agricultural Products |
Organiser |
Fermentation Research Center for Value Added Agricultural Products, Khon Kaen University |
Conference Place |
Kosa Hotel |
Province/State |
Khon Kaen, Thailand |
Conference Date |
29 August 2011 |
To |
31 August 2011 |
Proceeding Paper |
Volume |
4 |
Issue |
1 |
Page |
69 |
Editors/edition/publisher |
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Abstract |
Sweet sorghum stalks with the peel (6 to 20 mm in diameter and 6 mm in thickness) were used as low cost support materials for immobilization of Saccharomyces cerevisiae NP 01 to produce ethanol. To immobilize the yeasts, the sterilized stalks were mixed with yeast extract malt extract (YM) medium containing yeast cell concentration of 1×108 cells ml-1 under static condition at 30 oC for 24 h before use as an inoculum for ethanol production. The ethanol production medium was sweet sorghum juice containing 240 g l-1 of total sugar without any nutrient supplement. Continuous ethanol fermentation by the immobilized yeasts on the sweet sorghum stalk was carried out in a 780-ml tubular packed-bed bioreactor with different packed-bed volumes (30 and 50 %) at the dilution rate of 0.045 h-1. The results showed that the packed-bed volume did not affect the ethanol production efficiencies. At the steady state, the ethanol concentration (P), productivity (Qp) and yield (Yp/s) of both conditions were 56 g l-1, 2.5 g l-1 h-1 and 0.52 g g-1, respectively. The effects of dilution rates (0.013 and 0.022 h-1) at 30% bed volume were investigated in a double tubular packed-bed bioreactor. The results indicated that the lower dilution rate gave higher ethanol production efficiencies. The average P, Qp and Yp/s were 99.93±1.13 g l-1, 1.30±0.01 g l-1 h-1 and 0.47±0.02 g g-1, respectively. Moreover, the continuous system could be operated at least 432 h without any losses in the ethanol production efficiencies. The results obtained clearly demonstrated that sweet sorghum stalk is suitable as a support material for yeast cell immobilization. |
Author |
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Peer Review Status |
มีผู้ประเมินอิสระ |
Level of Conference |
นานาชาติ |
Type of Proceeding |
Abstract |
Type of Presentation |
Poster |
Part of thesis |
true |
Presentation awarding |
false |
Attach file |
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Citation |
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