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             Publication
Journal Publication
Research Title Efficient precipitation methods of inulinase from endophytic bacteria bacillus aquimaris isolated from jerusalem artichoke 
Date of Distribution 3 March 2017 
Conference
     Title of the Conference Engineering & Technology, Computer, Basic & Applied Sciences” (ECBA- 2017)  
     Organiser academic fora 
     Conference Place HI SEOUL YOUTH HOSTEL  
     Province/State seoul  
     Conference Date 3 March 2017 
     To 4 March 2017 
Proceeding Paper
     Volume
     Issue
     Page 13 
     Editors/edition/publisher International Journal of Applied and Physical Sciences (IJAPS) 
     Abstract Inulinase is an enzyme that hydrolyzes inulin to oligosaccharide used in the food industry. The endophytic bacteria, Bacillus aquimaris3.13 isolated from jerusalem artichoke, obtained inulinase when grown in luriabertani medium (LB broth) containing 1% inulin as the carbon and energy source with incubation temperature at 37°C for 20 hours and shaking speed of 150 rpm. The sample showed inulinase activities at 729 U after incubation at 55 °C for 20 min in the presence of inulin substrate. There were four methods for precipitation : ammonium sulfate, ethanol, butanol, and 2-steps of salt and alcohol. The best precipitated protein method was 50% of ethanol, giving 53% protein recovery (388 U activity). 
Author
575020118-2 Miss KANOKWAN CHANSODA [Main Author]
Science Master's Degree

Peer Review Status ไม่มีผู้ประเมินอิสระ 
Level of Conference นานาชาติ 
Type of Proceeding Full paper 
Type of Presentation Oral 
Part of thesis true 
Presentation awarding false 
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