| Research Title |
Efficient precipitation methods of inulinase from endophytic bacteria bacillus aquimaris isolated from jerusalem artichoke |
| Date of Distribution |
3 March 2017 |
| Conference |
| Title of the Conference |
Engineering & Technology, Computer, Basic & Applied Sciences” (ECBA- 2017) |
| Organiser |
academic fora |
| Conference Place |
HI SEOUL YOUTH HOSTEL |
| Province/State |
seoul |
| Conference Date |
3 March 2017 |
| To |
4 March 2017 |
| Proceeding Paper |
| Volume |
4 |
| Issue |
3 |
| Page |
13 |
| Editors/edition/publisher |
International Journal of Applied and Physical Sciences (IJAPS) |
| Abstract |
Inulinase is an enzyme that hydrolyzes inulin to oligosaccharide used in the food industry. The endophytic bacteria, Bacillus aquimaris3.13 isolated from jerusalem artichoke, obtained inulinase when grown in luriabertani medium (LB broth) containing 1% inulin as the carbon and energy source with incubation temperature at 37°C for 20 hours and shaking speed of 150 rpm.
The sample showed inulinase activities at 729 U after incubation at 55 °C for 20 min in the presence of inulin substrate. There were four methods for precipitation : ammonium sulfate, ethanol, butanol, and 2-steps of salt and alcohol. The best precipitated protein method was 50% of ethanol, giving 53% protein recovery (388 U activity). |
| Author |
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| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
0
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Publication
Journal Publication
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