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Journal Publication
Title of Article KT2 and RT2 modified antimicrobial peptides derived from Crocodylus siamensis Leucrocin I show activity against human colon cancer HCT-116 cells  
Date of Acceptance 11 July 2018 
Journal
     Title of Journal Environmental Toxicology and Pharmacology 
     Standard ISI 
     Institute of Journal European Journal of Pharmacology, 
     ISBN/ISSN  
     Volume 2018 
     Issue
     Month September
     Year of Publication 2018 
     Page 164-176 
     Abstract Conventional colon cancer treatments have been associated with side effects. Consequently, the discovery of novel effective and safe therapies is urgently needed. Hence, cationic antimicrobial peptides KT2 and RT2 were evaluated towards human colon cancer HCT-116 cells. The MTT assay indicated that both KT2 and RT2 exhibited anticancer activity with good therapeutic indices, and were found to be non-toxic to non-cancerous Vero cells. The IC50 values of KT2 were determined as 111.96 and 90.25 µg/mL while RT2 showed IC50 as 104.07 and 87.84 µg/mL after 12 and 24 h treatments, respectively. Moreover, KT2 and RT2 treatment caused a significant reduction in PI3K, AKT1 and mTOR mRNA expression levels, which resulted in suppression either of HCT-116 proliferation or migration. The mechanism involved in apoptosis induction were due to decreased Bcl-2 and XIAP and increased p53, cytochrome C, caspase-2, caspase-3, caspase-8, and caspase-9 mRNA expression levels. These effects increased the level of cell cycle associated gene p21 and decreased cyclin B1 and cyclin D1 expression. 
     Keyword Anticancer activity; Apoptosis; Colorectal cancer; Crocodile; Peptide 
Author
597020014-0 Mr. SURACHAI MAIJAROEN [Main Author]
Science Doctoral Degree

Reviewing Status มีผู้ประเมินอิสระ 
Status ตีพิมพ์แล้ว 
Level of Publication นานาชาติ 
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