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Journal Publication
Research Title Characterization and Classification of Proteases from the Digestive Organs in Different Sizes of Nile tilapia (Oreochromis niloticus, L)  
Date of Distribution 24 August 2018 
Conference
     Title of the Conference the 5th International Conference on Fisheries and Aquaculture (ICFA 2018) 
     Organiser Universitas Muhammadiyah Malang, Indonesia and Uva Wellassa University Sri Lanka 
     Conference Place Taj Samudra Colombo  
     Province/State Colombo, Sri Lanka 
     Conference Date 23 August 2018 
     To 24 August 2018 
Proceeding Paper
     Volume 2018 
     Issue
     Page 40 
     Editors/edition/publisher Prof. S.C. Jayamanne 
     Abstract Studies of proteolytic enzymes in Nile tilapia (Oreochromis niloticus, L.) contribute to a better understanding and improve protein utilization in feed. The investigation of proteolytic enzymes from digestive organs (stomach, proximal intestine, distal intestine and liver) using two different sizes of tilapia (6.44 ± 0.86 g. and 31.67 ± 2.31 g.). The effect of different pH (2.0-13.0) and incubated temperature (30, 45, 60 and 75°C) on protease activities using crude enzyme extract was measured by the increase in cleavage of short chain polypeptide which used azocasein as a substrate. Protease was classified by specific inhibitors. The protease activity of all organs in both sizes was highest at pH 9.0 and 45 °C. In addition, small fish showed highest acidic protease activity at pH 3.0. The protease activity of proximal intestine, distal intestine from both sizes and small fish liver were strongly inhibited by PMSF, TLCK and SBT, the serine protease inhibitors specifically for trypsin. Except in the protease activity of liver from the large fish was inhibited by EDTA (carboxypeptidase inhibitor). Meanwhile, pepstatin A, pepsin inhibitor, inhibited the acid protease activity in stomach extracts from both fish sizes. Results suggested that the present of proteases were differed among digestive organs. Knowledge gained from this study provided important information for further protease digestibility and feed formulation. 
Author
547030023-8 Miss THATSANEE ANUKOOLPRASERT [Main Author]
Agriculture Doctoral Degree
595030061-1 Mr. SATAPHON NONKHUKHETKHONG
Agriculture Master's Degree
557030013-2 Mr. KHAJORNKIAT SRINUANSOM
Agriculture Doctoral Degree
577030022-3 Mr. THANASORN RUKDONTRI
Agriculture Doctoral Degree

Peer Review Status มีผู้ประเมินอิสระ 
Level of Conference นานาชาติ 
Type of Proceeding Abstract 
Type of Presentation Oral 
Part of thesis true 
Presentation awarding false 
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