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Publication
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Research Title |
DETERMINATION OF THE CYTOTOXICITY AND ANTIOXIDANT ACTIVITY IN WILD EDIBLE MUSHROOM |
Date of Distribution |
19 November 2018 |
Conference |
Title of the Conference |
The 44th Congress on Science and Technology of Thailand (STT 44) |
Organiser |
The Science Society of Thailand under the Patronage of His Majesty the King Faculty of science, Chulalongkorn University |
Conference Place |
Bangkok International Trade & Exhibition Centre: BITEC |
Province/State |
Bangkok |
Conference Date |
29 October 2018 |
To |
31 October 2018 |
Proceeding Paper |
Volume |
1 |
Issue |
1 |
Page |
113 |
Editors/edition/publisher |
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Abstract |
Mushrooms are widely consumed in many countries. It has reported beneficial effects
for human health because they contain full of proteins, rich in vitamins and minerals. However,
some species are dangerous because they cause toxicity. Therefore, wild mushroom from
the local market in Khon Kaen that are popular in northeastern region of Thailand was subjected
to 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay for finding
a scientific answer to concerning about their relating to a toxin effect on macrophage (RAW
264.7) cell, human peripheral blood mononuclear cell (PBMC) and human red blood cell (hRBC).
The nucleotide sequences identification using DNA barcoding of nuclear ribosomal internal
transcribed spacer (ITS) was indicated that this mushroom identical to Amanita hemibapha
(Hed Ra York Laung). The antioxidant activity of A. hemibapha was evaluated by DPPH
scavenging assay compared to ascrorbic acid (Vit C). The results show that crude A. hemibapha
water extract (cAWE) at the concentration of 12.5 g/ml had antioxidant activity about 70% which
was a more than Vit C (20 %). Moreover, the MTT assay results show that cAWE at concentration
of 0.78-100 μg/ml had no cytotoxic effect on PBMCs and hRBC. However, the viability of RAW
264.7 cells was reduced to 65% after co-cultured with 50 μg/ml cAWE. At the concentration of
100 μg/ml, cAWE significantly reduced RAW 264.7 cells viability by 20% after 24 h treatment.
The collected data clearly demonstrate that although cAWE possess a great antioxidant activity,
but the possible risks to RAW 264.7 cells could be observed at the high concentration. Therefore,
experimental and also clinical evaluations are required to identify the possible side effects of
wild mushrooms. |
Author |
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Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
Level of Conference |
ชาติ |
Type of Proceeding |
Abstract |
Type of Presentation |
Poster |
Part of thesis |
true |
Presentation awarding |
false |
Attach file |
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Citation |
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