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Journal Publication
Research Title DETERMINATION OF THE CYTOTOXICITY AND ANTIOXIDANT ACTIVITY IN WILD EDIBLE MUSHROOM 
Date of Distribution 19 November 2018 
Conference
     Title of the Conference The 44th Congress on Science and Technology of Thailand (STT 44) 
     Organiser The Science Society of Thailand under the Patronage of His Majesty the King Faculty of science, Chulalongkorn University 
     Conference Place Bangkok International Trade & Exhibition Centre: BITEC 
     Province/State Bangkok 
     Conference Date 29 October 2018 
     To 31 October 2018 
Proceeding Paper
     Volume
     Issue
     Page 113 
     Editors/edition/publisher  
     Abstract Mushrooms are widely consumed in many countries. It has reported beneficial effects for human health because they contain full of proteins, rich in vitamins and minerals. However, some species are dangerous because they cause toxicity. Therefore, wild mushroom from the local market in Khon Kaen that are popular in northeastern region of Thailand was subjected to 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay for finding a scientific answer to concerning about their relating to a toxin effect on macrophage (RAW 264.7) cell, human peripheral blood mononuclear cell (PBMC) and human red blood cell (hRBC). The nucleotide sequences identification using DNA barcoding of nuclear ribosomal internal transcribed spacer (ITS) was indicated that this mushroom identical to Amanita hemibapha (Hed Ra York Laung). The antioxidant activity of A. hemibapha was evaluated by DPPH scavenging assay compared to ascrorbic acid (Vit C). The results show that crude A. hemibapha water extract (cAWE) at the concentration of 12.5 g/ml had antioxidant activity about 70% which was a more than Vit C (20 %). Moreover, the MTT assay results show that cAWE at concentration of 0.78-100 μg/ml had no cytotoxic effect on PBMCs and hRBC. However, the viability of RAW 264.7 cells was reduced to 65% after co-cultured with 50 μg/ml cAWE. At the concentration of 100 μg/ml, cAWE significantly reduced RAW 264.7 cells viability by 20% after 24 h treatment. The collected data clearly demonstrate that although cAWE possess a great antioxidant activity, but the possible risks to RAW 264.7 cells could be observed at the high concentration. Therefore, experimental and also clinical evaluations are required to identify the possible side effects of wild mushrooms. 
Author
595020006-5 Miss PRAKAYMARS PANUMARS [Main Author]
Science Master's Degree

Peer Review Status ไม่มีผู้ประเมินอิสระ 
Level of Conference ชาติ 
Type of Proceeding Abstract 
Type of Presentation Poster 
Part of thesis true 
Presentation awarding false 
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