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Publication
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| Title of Article |
Optimized models of xenobiotic-induced oxidative stress in HepG2 cells |
| Date of Acceptance |
24 April 2019 |
| Journal |
| Title of Journal |
Tropical Journal of Pharmaceutical Research |
| Standard |
SCOPUS |
| Institute of Journal |
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria |
| ISBN/ISSN |
1596-5996 |
| Volume |
18 |
| Issue |
5 |
| Month |
May |
| Year of Publication |
2019 |
| Page |
1001-1007 |
| Abstract |
Purpose: To evaluate the molecular impact of ethanol, sodium selenite, and tert-butyl hydroperoxide (TBHP) on oxidant-antioxidant balance in HepG2 cells to establish an optimized oxidative stress model of HepG2 cells.
Methods: HepG2 cells were treated with ethanol (10 - 500 mM) and sodium selenite (1 - 10 µM) for 24 and 48 h and with TBHP (50 - 200 µM) for 3 and 24 h, respectively. Biomarkers for cellular injury, ie, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and malondialdehyde (MDA), and for antioxidant system, i.e., superoxide dismutase (SOD), catalase (CAT), and total glutathione content, were determined.
Results: All treatments increased the levels of LDH, AST, ALT, and MDA but decreased SOD and CAT activities and the total glutathione content in HepG2 cells. Oxidative stress was induced by these oxidative stressors in HepG2 cells via oxidant-antioxidant imbalance, with TBHP (100 µM, 3 h) acting as a powerful oxidant based on the minimal time to induce oxidative stress. The antioxidants, ascorbic acid and gallic acid, improved oxidant-antioxidant imbalance against xenobiotic-induced oxidative stress in HepG2 cells.
Conclusion: These oxidative stress models are suitable for investigating the antioxidant and/or hepatoprotective potential of chemicals, including natural compounds. |
| Keyword |
Ethanol, Sodium selenite, Tert-butyl hydroperoxide, Oxidative stressor, Oxidant-antioxidant balance |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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