| Research Title |
Analysis of protein expression in sugarcane leaves in response to water-deficit stress using 2-dimensional gel electrophoresis |
| Date of Distribution |
9 April 2011 |
| Conference |
| Title of the Conference |
Proceedings of 17th National Genetic Conference, |
| Organiser |
ภาควิชาชีววิทยา คณะวิทยาศาสตร์ มหาวิทยาลับเชียงใหม่ ร่วมกับ สมาคมพันธุศาสตร์แห่งประเทศไทย |
| Conference Place |
โรงแรมอิมพีเรียลแม่ปิง |
| Province/State |
เชียงใหม่ |
| Conference Date |
18 April 2011 |
| To |
18 April 2011 |
| Proceeding Paper |
| Volume |
17 |
| Issue |
OP8 |
| Page |
99 |
| Editors/edition/publisher |
|
| Abstract |
Two-dimensional gel electrophoresis (2-DE) technique is wildly used for protein research. The objective of this work was to to use 2-DE coupled with analysis of gels for identification of proteins differentially expressed in response to water deficit stress. Sugarcane plants were planted and water stress imposed. Soil moisture content (SMC), Leaf water potential (LWP) and relative water content (RWC) indicated that sugarcane plants were under water deficit stress. Proteins were extracted from sugarcane leaves under water stress and adequate water supply and isolated in three replicated gels using 2-DE. Proteins were visualized by silver nitrate staining and the gels analyzed using Melanie 7 software. There were 9 down-regulated and 24 up- regulated proteins that displayed significant changes (p≤0.1-.05) in response to water deficit stress. Identification of the responsive proteins may provide insight into cellular mechanisms of drought tolerance in sugarcane. |
| Author |
|
| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
0
|
|
|
Publication
Journal Publication
ไทย