2012 ©
             Publication
Journal Publication
Research Title Immunorestoration of Lentinus polychrous mycelial extract on immunocompromised model in in Vitro 
Date of Distribution 1 March 2012 
Conference
     Title of the Conference 1st ASEAN PLUS THREE GRADUATE RESEARCH CONGRRESS 
     Organiser บัณฑิตวิทยาลัย, สำนักงานคณะกรรมการการอุดมศึกษา 
     Conference Place เชียงใหม่ 
     Province/State  
     Conference Date 1 March 2012 
     To 2 March 2012 
Proceeding Paper
     Volume
     Issue
     Page HS-255 - HS-259 
     Editors/edition/publisher  
     Abstract Lentinus polychrous, Thai local edible mushroom, was cultured under solid state fermentation. The ethanolic extract of their mycelia (LPME) was investigated for its in vitro effects in immune-restoration. Balb/cMal mice were pre-treated with cyclophosphamide (Cyc) for four days prior to removal of their spleens for splenocyte cultivation. The cytotoxic effect of LPME against Cyc-treated splenocytes was determined. In addition, the effect of LPME on Cyc-treated splenocyte proliferation was evaluated in the presence of T-cell and B-cell inducing mitogens, phytohemagglutinin (PHA) and poke weed mitogen (PWM), respectively. The results showed that the Cyc treatment decreased size of spleen and viable cell numbers of splenocytes compared with Cyc untreatment. The LPME exhibited cytotoxicity against Cyc-treated splenocytes with IC50 of 76.12 ± 8.24 µg/ml. In the presence of PHA and PWM, the LPME induced the proliferation of the Cyc-treated cells significantly. These results indicate that the LPME can restore the proliferation of both immunosuppressed splenocytes. 
Author
507150003-1 Mrs. NIRAMAI FANGKRATHOK [Main Author]
Pharmaceutical Sciences Doctoral Degree

Peer Review Status มีผู้ประเมินอิสระ 
Level of Conference นานาชาติ 
Type of Proceeding Full paper 
Type of Presentation Poster 
Part of thesis true 
Presentation awarding true 
     Award Title Excellent poster presentor 
     Type of award รางวัลด้านวิชาการ วิชาชีพ 
     Organiser สำนักงานคณะกรรมการการอุดมศึกษา 
     Date of awarding 2 มีนาคม 2555 
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