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Publication
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| Research Title |
A peptide chimera comprised of the bovine lactoferrin antimicrobial peptides lactoferricin and lactoferrampin exhibits potent killing activity against Burkholderia pseudomallei |
| Date of Distribution |
25 October 2012 |
| Conference |
| Title of the Conference |
5th Annual Symposium on Host Defence Peptides |
| Organiser |
The Division of Molecular Host Defence of Utrecht University and the section of Oral Biochemistry of the Academic Centre for Dentistry Amsterdam |
| Conference Place |
Utrecht University |
| Province/State |
Utrech, The Netherlands |
| Conference Date |
25 October 2012 |
| To |
25 October 2012 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
- |
| Editors/edition/publisher |
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| Abstract |
Burkholderia pseudomallei is the etiological agent of melioidosis, which is a life-threatening infectious disease in Southeast Asia and Northern Australia. Successful treatment of melioidosis patients is difficult because the pathogen is inherently resistant to most commonly used antibiotics, including β-lactams, aminoglycosides, macrolides and polymyxins. The current treatment requires an initial intensive therapy with Ceftazidime (CAZ) administered parenterally for up to 4 weeks followed with an eradication therapy for up to 6 month with a cocktail of antibiotics administered orally. Lactoferricin and lactoferrampin are two antimicrobial stretches of the innate immunity factor bovine lactoferrin, which have been led to the development a series of antimicrobial peptides with broad spectrum activity against a range of Gram-positive and Gram-negative bacteria. In this study we evaluated the antimicrobial activity of heterodimers of peptides from both stretches, against B. pseudomallei in comparison to CAZ, using 7 strains of this bacterium including a CAZ-resistant strain. As the major route of infection is the respiratory tract the cytotoxicity of the peptides was examined using the lung epithelium cell line A549. The antimicrobial potency of the individual peptides was dose dependent and differed between the bacterial isolates. In all cases the heterodimer LFchimera displayed higher killing activity than their constituent peptides. Time-kill experiments showed LFchimera at a concentration as low as 5 μM can kill B. pseudomallei within 2 h. Also B. pseudomallei 979b, shown to be resistant to CAZ, was highly susceptible to LFchimera, though a higher concentration was needed (10 μM). At these concentrations of LFchimera we found no reduction of viability of the A549 cells. These data indicate that LFchimera might be potential as alternative therapeutic agents against B. pseudomallei. |
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| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
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