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Publication
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| Research Title |
Effect of Culture Media on Fertilization Rate in Bovine and Caprine Embryos Production |
| Date of Distribution |
21 January 2012 |
| Conference |
| Title of the Conference |
the 3rd International Conference on Environmental and Rural Development |
| Organiser |
International society of enviromental and rural development |
| Conference Place |
Khon Kaen University, Thailand |
| Province/State |
Khon Kaen |
| Conference Date |
21 January 2012 |
| To |
22 January 2012 |
| Proceeding Paper |
| Volume |
3 |
| Issue |
- |
| Page |
36 |
| Editors/edition/publisher |
Prof. Dr.Machito MIHARA |
| Abstract |
The objective of this experiment was to determine the effect of culture media (Tyrode albumin lactate pyruvate-In vitro fertilization-Synthetic oviduct fluid; TALP-IVF-SOF and SAGE mediaTM ) on fertilization rate in bovine and caprine embryos production. Thai crossbred bovine ovaries (n=40) were collected at a local abattoir located in Khon Kaen municipality. Caprine ovaries (n=22) were collected by the surgery method at the Department of Animal Science, Faculty of Agriculture, Khon Kaen University. In order to compare the culture media, oocytes were recovered and randomly cultured in TALP-IVF-SOF and SAGE mediaTM during in vitro oocytes maturation and fertilization as described by the standard protocols for in vitro maturation, in vitro fertilization, and in vitro culture respectively. Oocytes, obtained from ovaries with corpus luteum (CL) and without CL, were recovered and determined as recovery rate prior in vitro culture. The results revealed that the recovery rates of bovine and caprine oocytes were not significantly different between ovaries with CL (58.5 and 27.6%) without CL (43.5 and 29.4%), bovine oocytes had significantly more layers of cumulus than caprine oocytes. IVF and IVC, developed from two cells to blastocyst stage, derived from SAGE mediaTM were better than that of TALP-IVF-SOF.
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย