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Publication
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| Title of Article |
Evaluation of Ovarian Follicular Health with the Markers of Endothelial |
| Date of Acceptance |
7 September 2012 |
| Journal |
| Title of Journal |
Chiang Mai University Journal of Natural Sciences (CMUJ of Nat.Sci.) |
| Standard |
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| Institute of Journal |
Chiang Mai University |
| ISBN/ISSN |
1685-1994 |
| Volume |
2012 |
| Issue |
11 |
| Month |
September |
| Year of Publication |
2012 |
| Page |
416-418 |
| Abstract |
The aim of the study was to determine the quantitative vascularity of the bovine preovulatory
follicle using Factor VIII immunolocalization associated with the follicular health. Eight
mature Thai native crossbred beef cows received two injections (11 day apart) of a PGF analogue
to synchronize estrus. The study was conducted to test the marker of endothelial cell (Factor
VIII) and granulosa cell (Pyknotic nucleus) for follicular health evaluation. Bovine preovulatory
follicles (n=32) were obtained on day 6 of the estrous cycle from abattoir located in Khon Kaen
provine. Follicles were classified by size as 3-6, 7-10, and >10 mm in diameter and by morphology
as healthy and unhealthy. Ovarian tissue samples were fixed and paraffin-embedded prior
immunohistochemistry. Factor VIII (Sigma, USA) and Pyknotic nuclei were applied as marker
of endothelial cell in the theca and granulosa cell respectively. Quantitative and qualitative
analysis of follicular health was determined with immunohistological assay with the aid of image
analysis (Media Cybernetics, Inc., Silver Spring, USA). Pyknotic nuclei in unhealthy follicles were
significantly greater than that of healthy follicles (P<0.05). Average follicular capillary area density,
and capillary number density were greater (P<0.01) in healthy follicles (4.60% and 0.0027 no./
μm2) compared with unhealthy follicles (1.76% and 0.0011 no./μm2). However, area per capillary
was not different between healthy (17.32 μm2/no.) and unhealthy follicles (14.13 μm2/no.). These
studies indicated that ovarian follicular health could be precisely evaluated with Factor VIII and
Pyknotic nuclei immunohistochemistry. In addition, the study supported the relationship between
the follicular health and vascularity during follicular development of the estrous cycle. |
| Keyword |
Bovine, Factor VIII, Follicular health, Pyknotic nuclei, Vascularity |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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