| Research Title |
Evaluation of ovarian follicular health with the markers of endothelial and granulosa cells |
| Date of Distribution |
9 January 2011 |
| Conference |
| Title of the Conference |
International Conference on the Role of Agriculture and Natural Resources on Global Change (ANGC 2011) |
| Organiser |
Faculty of Agriculture, Chiang Mai University, Thailand |
| Conference Place |
Imperial Mae Ping Hotel |
| Province/State |
Chiang Mai, Thailand |
| Conference Date |
7 January 2013 |
| To |
7 January 2013 |
| Proceeding Paper |
| Volume |
2011 |
| Issue |
- |
| Page |
79 |
| Editors/edition/publisher |
Chiang Mai University |
| Abstract |
The aim of the study was to determine the quantitative vascularity of the bovine preovulatory follicle using Factor VIII immunolocalization associated with the follicular health. Eight mature Thai native crossbred beef cows received two injections (11 day apart) of a PGF analogue to synchronize estrus. The study was conducted to test the marker of endothelial cell (Factor VIII) and granulosa cell (Pyknotic nuclease) for follicular health evaluation. Bovine preovulatory follicles (n=32) were obtained on day 6 of the estrous cycle from abattoir located in Khon Kaen provine. Follicles were classified by size as 3-6, 7-10, and >10 mm in diameter and by morphology as healthy and unhealthy. Ovarian tissue samples were fixed and paraffin-embedded prior immunohistochemistry. Factor VIII (Sigma, USA) and Pyknotic nuclei were applied as marker of endothelial cell in the theca and granulosa cell respectively. Quantitative and qualitative analysis of follicular health was determined with immunohistological assay with the aid of image analysis (Media Cybernetics, Inc., Silver Spring, USA). Pyknotic nuclei in unhealthy follicles were significantly greater than that of healthy follicles (P<0.05). Average follicular capillary area density, and capillary number density were greater (P<0.01) in healthy follicles (4.60% and 0.0027 no./µm2) compared with unhealthy (1.76% and 0.0011 no./µm2). However, area per capillary was not different between healthy (17.32 µm2/no.) and unhealthy follicles (14.13 µm2/no.). These studies indicated that ovarian follicular health could be precisely evaluated with Factor VIII and Pyknotic nuclei immunohistochemistry. In addition, the study supported the relationship between the follicular health and vascularity during follicular development of the estrous cycle. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Presentation awarding |
true |
| Award Title |
Best Oral Presentation Award |
| Type of award |
รางวัลด้านวิชาการ วิชาชีพ |
| Organiser |
Faculty of Agriculture, Chiang Mai University, Thailand |
| Date of awarding |
9 พฤศจิกายน 2554 |
| Attach file |
|
| Citation |
0
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Publication
Journal Publication
ไทย