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Publication
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| Title of Article |
Multiplex PCR for detection of superantigenic toxin genes in methicillin-sensitive and methicillin-resistant Staphylococcus aureus isolated from patients and carriers of a hospital in Northeast Thailand |
| Date of Acceptance |
20 June 2013 |
| Journal |
| Title of Journal |
The Southeast Asian Journal of Tropical Medicine and Public Health |
| Standard |
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| Institute of Journal |
Faculty of Tropical Medicine, Mahidol University, Thailand |
| ISBN/ISSN |
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| Volume |
44 |
| Issue |
4 |
| Month |
July |
| Year of Publication |
2014 |
| Page |
660-671 |
| Abstract |
The aims of this study were to develop multiplex PCR for simultaneous detection of five superantigenic toxin genes (sea, seb, sec, sed and tst-1) in Staphylococcus aureus isolated from 149 clinical samples and nasal swabs from 201 healthy subjects in Thailand, and to compare prevalence and expression of those genes between methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA). The sensitivity of multiplex PCR was 103 CFU/ml (60 CFU/PCR reaction) for DNA templates extracted by both boiling and extraction methods. S. aureus strains from patients (65%) harbored more superantigenic toxin genes than healthy subjects (54%). MRSA (80%) isolated from patients harbored more superantigenic toxin genes than MSSA (52%). Sea was the most frequently found gene in S. aureus strains from patients and carriers. MRSA isolates harbored sea and produced SEA more frequently than MSSA isolates (p< 0.05) and MRSA isolates (59%) from blood samples consisted of a higher number of superantigenic toxin producers than MSSA (9%) (p< 0.05). More S. aureus strains isolated from patients with severe septicemia contained superantigenic toxin genes (94%) and produced toxins (82%) than those from non-severe patients (64% and 57%, respectively).
The multiplex PCR method described here offers a reliable tool for simultaneous detection of various staphylococcal toxin genes. |
| Keyword |
multiplex PCR, superantigenic toxin, methicillin-resistant S. aureus, methicillin-sensitive S. aureus, patients, nasal carriers, Thailand |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย