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Publication
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| Research Title |
Bile canalicular alteration and bile secretory defect in acute and chronic Opisthorchis viverrini-infected hamsters |
| Date of Distribution |
12 May 2013 |
| Conference |
| Title of the Conference |
Asian Parasites by Asian Parasitologists (APAP) : Helminthiases in Greater Mekong Subregion |
| Organiser |
Department of Parasitology, Faculty of Medicine Khon Kaen University, Thailand |
| Conference Place |
Department of Parasitology, Faculty of Medicine Khon Kaen University, Thailand |
| Province/State |
Thailand |
| Conference Date |
12 May 2013 |
| To |
12 May 2013 |
| Proceeding Paper |
| Volume |
1 |
| Issue |
1 |
| Page |
23 |
| Editors/edition/publisher |
Lakhanawan Charoensuk1,2,3, Porntip Pinlaor3,4, Wunnee Chaijaroonkhanarak5, Puangrat Yongvanit3,6, Chawalit Pairojkul3,7, Yukifumi Nawa8, Somchai Pinlaor1,2,3* |
| Abstract |
Opisthorchis viverrini infection induces inflammation and liver injury leading to increase periductal fibrosis with time. However, it's remain unclear whether these changes have effect on the original of bile duct changes. The aim of this study was to investigate the alterations of bile canaliculi (BC) and biliary physiology in hamster liver infected with O. viverrini during acute and chronic phases. In O. viverrini infected groups, electron microscopy revealed that BC showed tortuous course, dilatation of canalicular lumen and decreased the density of microvilli compared to normal control. The mRNA expression of FXR and Shp-1 genes significantly increased, but expression of Mrp2 and Ntcp significantly decreased in both acute and chronic of O. viverrini-infected groups compared to control group. A significantly decrease expression of BSEP, Ntcp, Cyp7a1, Cyp27a1 and CD10 genes was observed only in acute infection. In addition, CD10 staining for a canalicular pattern was significantly lower level than in the normal group. In contrast, the fibrotic markers including mRNA expressions of collagen I and III, protein expression of fibroblast specific marker α-SMA and serum hydroxyproline content were significantly higher levels than that of control. Increase of fibrotic markers was associated with the thickness of periductal fibrosis. In conclusion, O. viverrini infection induces BC alterations and decreases bile flow, bile acid uptake transporters and bile acid biosynthesis in relation to periductal fibrosis. The study may provide an insight information of biliary changes in opisthorchiasis- associated disease. |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
true |
| Award Title |
The outstanding poster presentation |
| Type of award |
รางวัลด้านวิชาการ วิชาชีพ |
| Organiser |
Department of Parasitology, Faculty of Medicine Khon Kaen University, Thailand |
| Date of awarding |
12 พฤษภาคม 2556 |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย