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Publication
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| Research Title |
Bactericidal activity of LFchimera, a heterodimer comprised of two bovine lactoferrin antimicrobial peptides, against Burkholderia pseudomallei |
| Date of Distribution |
19 September 2013 |
| Conference |
| Title of the Conference |
The 7th World Melioidosis Congress 2013 (WMC 2013) |
| Organiser |
The Mahidol Oxford Tropical Medicine ResearchUnit (MORU) and the Faculty of Tropical Medicine, Mahidol University |
| Conference Place |
Royal Orchid Sheraton Hotel |
| Province/State |
Bangkok, Thailand |
| Conference Date |
18 September 2013 |
| To |
20 September 2013 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
- |
| Editors/edition/publisher |
- |
| Abstract |
Burkholderia pseudomallei, the causative agent of melioidosis, has developed resistance to an
increasing list of antibiotics, demanding a search for novel agents. Lactoferricin and lactoferrampin are
two antimicrobial domains of lactoferrin with a broad spectrum of antimicrobial activity. A hybrid
peptide (LFchimera) containing lactoferrampin (LFampin265-284) and a part of lactoferricin (LFcin17-30) has strikingly higher antimicrobial activities compared to the individual peptide s. In this study, the
antimicrobial activities of this chimeric construct (LFchimera1), as well as of another one containing
LFcin17-30 and LFampin268-284, a shorter fragment of LFampin265-284 (LFchimera2), and the
constituent peptides were tested against 7 isolates of B. pseudomallei and compared to the preferential
antibiotic ceftazidime (CAZ). All isolates including B. pseudomallei 979b shown to be resistant to CAZ,
at a density of 105 CFU/ml, could be killed by 5-10 μM of LFchimera1 within 2 h, while the other
peptides as well as the antibiotic CAZ only inhibited the B. pseudomallei strains resulting in an
overgrowth in 24 h. These data indicate that LFchimera1 could be considered for development of
therapeutic agents against B. pseudomallei. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย