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Publication
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| Research Title |
Epstein-Barr Virus as a Risk Factor of Cervical Cancer by Association with High Risk Human Papillomavirus Episome |
| Date of Distribution |
26 June 2013 |
| Conference |
| Title of the Conference |
International Conference in Medicine and Public Health (ICMPH) 2013 |
| Organiser |
Faculty of Medicine Siriraj Hospital, Mahidol University Supporting Organization, The Ministry of Public Health, The Rockefeller Foundation |
| Conference Place |
Faculty of Medicine Siriraj Hospital, Mahidol University |
| Province/State |
Bangkok, Thailand |
| Conference Date |
24 June 2013 |
| To |
28 June 2013 |
| Proceeding Paper |
| Volume |
65 |
| Issue |
3 |
| Page |
RS-032 |
| Editors/edition/publisher |
Siriraj medicine journal |
| Abstract |
Objective(s): Epstein-Barr Virus (EBV) is an etiologic factor in multiple types of cancer that primarily develop in lymphocytes and epithelial cells. To explore the role of EBV in high risk human papillomavirus (HR-HPV) induced carcinogenesis, the correlation of EBV infection with HPV physical status was investigated.
Material(s) and Method(s): Cervical tissues consisting of no squamous intraepithelial lesion (noSIL, 38 cases), low grade SIL (LSIL, 85 cases), high grade SIL (HSIL, 81 cases) and cervical carcinoma (CA, 38 cases) were extracted for RNA and DNA using Trizol. DNA was used to detect EBV and HPV by PCR, dot blot hybridization assay and reverse line blot hybridization assay. The physical status of HR-HPV was investigated by amplification of papillomavirus oncogene transcripts assay using the extracted RNA.
Results: EBV detection rate in HPV positive cases increased with increasing severity of cervical lesion: noSIL (23.7%), LSIL (29.4%) and HSIL (48.2%). Co-detection of HPV and EBV was significantly higher in HSIL+CA (36.13%) than noSIL+LSIL (13.82%). Physical status could be investigated in 60 HR-HPV cases (12 cases of noSIL+LSIL and 48 cases of HSIL+CA). Only episome was found in noSIL+LSIL. In HSIL+CA, episome was found significantly more often in EBV-HPV co-infections (69.2%) than HPV infection alone (31.8%) whereas integration was found significantly more often in HPV infection alone (68.2%) than EBV-HPV co-infections (30.8%).
Conclusion: These results reveal novel information of EBV as a risk factor correlated with HPV episome and suggest that EBV-HPV co-infections in cervix may modulate cervical cancer progression.
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| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย