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Publication
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| Research Title |
EFFECT OF NANOENCAPSULATED CURCUMIN ON PERIDUCTAL FIBROSIS RESOLUTION AND BILE CANALICULI ALTERATION IN OPISTHORCHIS VIVERRINI-INFECTED HAMSTERS AFTER PRAZIQUANTEL TREATMENT |
| Date of Distribution |
11 December 2013 |
| Conference |
| Title of the Conference |
Joint International Tropical Medicine Meeting 2013 |
| Organiser |
Faculty of Tropical Medicine, Mahidol University |
| Conference Place |
Centara Grand & Bangkok Convention Centre at CentralWorld |
| Province/State |
Bangkok |
| Conference Date |
11 December 2013 |
| To |
13 December 2013 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
- |
| Editors/edition/publisher |
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| Abstract |
Opisthorchis viverrini infection induces inflammation and liver injury leading to increase periductal fibrosis with time and increase a risk for cholangiocarcinoma (CCA) development. Although praziquantel (PZQ) treatment would be valuable for O. viverrini elimination; however, periductal fibrosis usually remains for a long time after treatment at chronic infection. To reduce periductal fibrosis induced by O. viverrini infection, encapsulated curcumin was used to enhance fibrolysis of fibrotic tissue after praziquantel treatment in O. viverrini-infected hamsters. Forty-eight hamsters were divided into 4 groups: (1) normal hamsters, (2) O. viverrini-infected hamsters (OV), (3) OV treated with praziquantel (OP), and (4) OV+PZQ and treated with encapsulated curcumin at 50 mg/kg BW (OPC). Each experimental group was divided into two sup-group, acute (1 month) and chronic (3 months) post-infection. Encapsulated curcumin was orally administered daily for one month after PZQ-treatment. Acute and chronic groups showed similar results. Picrosirius red staining revealed that encapsulated curcumin reduced periductal fibrosis. The changes were related to the reduction of the expression of fibrotic markers (Matrix metalloproteinase (MMP) 9, collagen I, tissue inhibitor of matrix metalloproteinase (TIMP) 1, TIMP3, alpha-smooth muscle actin (a-SMA) and the increase of MMP2. Moreover, bile canaliculi feature was recovered by encapsulated curcumin treatment as demonstrated by the increasing of Neprilysin (CD10) and Multidrug resistance-associated protein 2 (Mrp2) expressions at the transcription and translation levels. In conclusion, encapsulated curcumin is a promising chemopreventive agent that can reduce periductal fibrosis and improve bile calicular structure after praziquantel treatment in opisthorchiasis which may be useful to reduce risk factor of CCA. |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
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