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Publication
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| Title of Article |
Rapid detection and differentiation of Clonorchis sinensis and Opisthorchis viverrini eggs in human fecal samples using a duplex real-time fluorescence resonance energy transfer PCR and melting curve analysis |
| Date of Acceptance |
21 December 2011 |
| Journal |
| Title of Journal |
Parasitology Research |
| Standard |
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| Institute of Journal |
Springer-Verlag |
| ISBN/ISSN |
0932-0113 |
| Volume |
1 |
| Issue |
11 |
| Month |
July |
| Year of Publication |
2012 |
| Page |
89-96 |
| Abstract |
We developed a single step duplex real-time fluorescence
resonance energy transfer (FRET) PCR merged
with melting curve analysis for the fast detection and differentiation
of Clonorchis sinensis and Opisthorchis viverrini
eggs in human fecal samples. Two species of mitochondrial
NADH dehydrogenase subunit 2 (nad2) DNA elements, the
165-bp nad2 product of C. sinensis and the 209-bp nad2
product of O. viverrini, were amplified by species-specific
primers, and the fluorescence melting curve analyses were
generated from hybrid of amplicons and two pairs of
species-specific fluorophore-labeled probes. By their different
fluorescence channels and melting temperatures, both C.
sinensis and O. viverrini eggs in infected human fecal samples
were detected and differentiated with high (100%)
sensitivity and specificity. Detection limit was as little as a
single C. sinensis egg and two O. viverrini eggs in 100 mg
of fecal sample. The assay could distinguish the DNA of
both parasites from the DNA of negative fecal samples and
fecal samples with other parasitosis, as well as from the
well-defined genomic DNA of human leukocytes and other |
| Keyword |
Clonorchis sinensis, Opisthorchis viverrini, real-time PCR, detection, differentiation, human stool |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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