| Research Title |
In Vitro and In Vivo Evaluation of Oxidative Stress Status and GABA-T Inhibitory Effects of Clitoria Ternatea Flowers in Hippocampus and Cerebral Cortex of Epileptic Mice Induced by Pentylenetetrazole |
| Date of Distribution |
5 February 2014 |
| Conference |
| Title of the Conference |
การประชุมวิชาการประจำปี 2557 ครั้งที่ 15 สมาคมประสาทวิทยาศาสตร์ ภาคตะวันออกเฉียงเหนือ |
| Organiser |
สมาคมประสาทวิทยาศาสตร์ ภาคตะวันออกเฉียงเหนือ |
| Conference Place |
ห้องประชุมมิตรภาพ ,ห้องประชุมมอดินแดง ชั้น 3 อาคารเรียนรวม คณะแพทยศาสตร์ มหาวิทยาลัยขอนแก่น |
| Province/State |
ขอนแก่น |
| Conference Date |
5 February 2014 |
| To |
7 February 2014 |
| Proceeding Paper |
| Volume |
8 |
| Issue |
4 |
| Page |
116-117 |
| Editors/edition/publisher |
|
| Abstract |
Introduction and objective: Due to the
crucial role of oxidative stress and GAB
Aergic system on the pathophysiology of
epilepsy and the beneficial effects of the
extract to improve seizure and memory
impairment, we aimed to determine both
in vitro and in vivo effect of C.ternatea on
oxidative stress status and GABAergic
function in cerebral cortex and hippocampus
of epileptic.
Materials and methods: The extract of C.
ternatea flowers was prepared via decoction
method. The yielded extract was lyophilized
as powder. The final extract contained totalphenolic compound at concentration of
71.916 + 1.816 mg/LGAE/mg extract. Then,
it was determined antioxidant using DPPH
and FRAP assays. To investigate the in vivo
effect of this plant extract, male ICR mice
were grouped into various groups as following;
1) control 2) vehicle 3) C. ternatea
extract 200 mg/kg 4) C. ternatea extract
400 mg/kg 5) C. ternatea extract 800 mg/
kgBW. The animals were orally given the
assigned substances for 14 days. Then, they
were injected PTZ at dose of 75 mg/kgBW
via intraperitoneal route. 24 hours later, all
animals were sacrificed, cerebral cortex and
hippocampus were collected to determine lipid peroxidation product (MDA) level and
GABA-T activity.
Results: The antioxidant activities of C.
ternatea flowers extract via DPPH and
FRAP assays were 174.83 ± 1.790 μg/ml
and 311.333 + 16.971 μM L-ascorbic acid
equivalence/mg extract, respectively while
the IC50 of GABA-T inhibition was 229.692
± 2.457 μg/ml. In addition, it was found
that all dose of C. ternatea flowers extract
showed the significant decreased MDA
level in hippocampus while C. ternatea
flowers extract at doses of 400 and 800 mg/
kgBW showed the significant decreased of
MDA level only in cerebral cortex. In addition,
the suppression effect of the extract
on GABA-T in cerebral cortex was also observed in cerebral cortex of epileptic mice
while the suppression effect on GABA-T in
hippocampus was observed only in epileptic
mice subjected to 200 mg/kgBW. Conclusion: Both in vitro and in vivo data
showed the antioxidant and GABAI effects
of C. ternatea flowers. These data suggest
that the extract might protect against brain
damage and over excitation in epilepsy.
Thus, it is worth for development as health
product to protect against epilepsy However,
the toxicity, behavior together with
biochemical and electrophysiology studies
are required before moving to clinical trial. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
true |
| Award Title |
Boehringer Ingelheim North Eastern Neuroscience Award |
| Type of award |
รางวัลด้านวิชาการ วิชาชีพ |
| Organiser |
สมาคมประสาทวิทยาศาสตร์ ภาคตะวันออกเฉ๊ยงเหนือ |
| Date of awarding |
6 กุมภาพันธ์ 2557 |
| Attach file |
|
| Citation |
0
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Publication
Journal Publication
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