| Research Title |
Starvation response, biofilm formation and drug tolerance in Burkholderia pseudomallei |
| Date of Distribution |
18 September 2013 |
| Conference |
| Title of the Conference |
7th World melioidosis congress 2013 |
| Organiser |
The Mahidol-Oxford Tropical Medicine Research Unit (MORU) |
| Conference Place |
Royal Orchid Sheraton Hotel |
| Province/State |
Bangkok, Thailand |
| Conference Date |
18 September 2013 |
| To |
20 September 2013 |
| Proceeding Paper |
| Volume |
7 |
| Issue |
1 |
| Page |
159 |
| Editors/edition/publisher |
The Mahidol-Oxford Tropical Medicine Research Unit (MORU) |
| Abstract |
Burkholderia pseudomallei is the causative agent of melioidosis, which can form biofilms and microcolonies both in vivo and in vitro. One of the hallmark characteristics of the biofilm-forming bacteria is that they can be up to 1,000 times more resistant to antibiotics than their free-living counterpart. Bacteria also become highly tolerant to antibiotics when nutrients are limited. One of the most important causes of starvation induced tolerance in vivo is biofilm growth. However, the correlation among starvation response, biofilm formation and drug tolerance in B. pseudomallei has never been reported. Therefore, this study aims to determine the effect of starvation on drug susceptibility of B. pseudomallei in both planktonic and biofilm forms in vitro. The biofilm formation of B. pseudomallei in starvation and enrich conditions was also evaluated by a modified microtiter-plate test. Six isolates of ceftazidime (CAZ)-susceptible B. pseudomallei were used. The drug susceptibility of planktonic B. pseudomallei in starvation and enrich conditions was determined by broth microdilution method. The Calgary biofilm device was used to determine the drug susceptibility of B. pseudomallei in biofilm form. The results showed that the minimum inhibitory concentrations of CAZ against B. pseudomallei in starvation were less than those in enrich condition while the minimum bactericidal concentrations of CAZ in starvation were higher. The drug susceptibilities of B. pseudomallei biofilm in both enrich and starvation conditions were significantly higher than those of planktonic cells. Moreover, the quantification of biofilm formation by B. pseudomallei in starvation was higher than that in enrich condition. These data indicate that the starvation could induce biofilm formation of B. pseudomallei and had effect on drug susceptibility compared with enrich condition. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
0
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Publication
Journal Publication
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