2012 ©
Journal Publication
Date of Distribution 22 March 2014 
     Title of the Conference 7th General Assembly and International Conference of Asian Pacific Organization for Cancer Prevention (2014 APOCP) 
     Organiser Asian Pacific Organization for Cancer Prevention (APOCP) 
     Conference Place Academia Sinica, Taipei, Taiwan 
     Province/State Taiwan, Republic of China 
     Conference Date 20 March 2014 
     To 23 March 2014 
Proceeding Paper
     Volume 2014 (electronic abstract file) 
     Issue F04-4 (electronic abstract file) 
     Page F04-4 (electronic abstract file) 
     Editors/edition/publisher Asian Pacific Organization for Cancer Prevention 
     Abstract The overexpression of p16INK4a is a key feature in oncogenic transformation caused by high-risk human papillomavirus (HR-HPV) and is used as a biomarker for cervical cancer progression. The relevance of HR-HPV infection in cervical and anal cancer is well established and HPV genomes are often detected in oral cancers, but it is unclear whether HPV has a specific oncogenic role. The relationship between HPV -positive oral squamous cell carcinoma (OSCC) and the expression of p16INK4a is controversial and still needed to elucidate. To understand the patterns of p16INK4a expression and their association with HPV infection in OSCC, this study investigated p16INK4a expression in formalin-fixed paraffin-embedded (FFPE) tissue of 148 OSCC and 6 cases of no oral squamous intraepithelial lesion (no SIL) by immunohistochemical staining (IHC). The expression patterns of p16INK4a positive cells were characterized by grading percentage including grade 0 (0%), grade 1 (1-10%), grade 2 (11-40%) and grade 3 (41-100%) using at least 500 cells/case. DNA was extracted from 5 sections of all FFPE samples and used for HPV DNA detection by polymerase chain reaction. The result showed that HPV prevalence was 16.89% (25/148) in OSCC and 0% (0/6) in no-SIL group. 56/148 of OSCC (37.84%) were p16INK4a positive and the patterns of p16INK4a expression were characterized to grade 1 (9 cases), grade 2 (15 cases) and grade 3 (32 cases). 1/6 of no-SIL (16.67%) was p16INK4a positive and classified as grade 3. p16INK4a expression in OSCC was found in 48% of HPV positive cases and 35.77% of HPV negative cases. No association between p16INK4a expression and HPV infection in OSCC. This result demonstrated that p16INK4a expression in OSCC was found in different patterns and not limited to HPV infection and suggested that p16INK4a expression may not be a perfect surrogate marker for HPV status in OSCC. 
517070025-7 Mr. PIYAWUT SWANGPHON [Main Author]
Medicine Doctoral Degree

Peer Review Status มีผู้ประเมินอิสระ 
Level of Conference นานาชาติ 
Type of Proceeding Abstract 
Type of Presentation Oral 
Part of thesis true 
Presentation awarding false 
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