| Research Title |
P16INK4A EXPRESSION PATTERNS IN ORAL SQUAMOUS CELL CARCINOMA AND THEIR ASSOCIATION WITH HUMAN PAPILLOMAVIRUS INFECTION |
| Date of Distribution |
22 March 2014 |
| Conference |
| Title of the Conference |
7th General Assembly and International Conference of Asian Pacific Organization for Cancer Prevention (2014 APOCP) |
| Organiser |
Asian Pacific Organization for Cancer Prevention (APOCP) |
| Conference Place |
Academia Sinica, Taipei, Taiwan |
| Province/State |
Taiwan, Republic of China |
| Conference Date |
20 March 2014 |
| To |
23 March 2014 |
| Proceeding Paper |
| Volume |
2014 (electronic abstract file) |
| Issue |
F04-4 (electronic abstract file) |
| Page |
F04-4 (electronic abstract file) |
| Editors/edition/publisher |
Asian Pacific Organization for Cancer Prevention |
| Abstract |
The overexpression of p16INK4a is a key feature in oncogenic transformation caused
by high-risk human papillomavirus (HR-HPV) and is used as a biomarker for cervical
cancer progression. The relevance of HR-HPV infection in cervical and anal cancer is
well established and HPV genomes are often detected in oral cancers, but it is unclear
whether HPV has a specific oncogenic role. The relationship between HPV -positive
oral squamous cell carcinoma (OSCC) and the expression of p16INK4a is controversial
and still needed to elucidate. To understand the patterns of p16INK4a expression and
their association with HPV infection in OSCC, this study investigated p16INK4a
expression in formalin-fixed paraffin-embedded (FFPE) tissue of 148 OSCC and 6
cases of no oral squamous intraepithelial lesion (no SIL) by immunohistochemical
staining (IHC). The expression patterns of p16INK4a positive cells were characterized
by grading percentage including grade 0 (0%), grade 1 (1-10%), grade 2 (11-40%) and
grade 3 (41-100%) using at least 500 cells/case. DNA was extracted from 5 sections of
all FFPE samples and used for HPV DNA detection by polymerase chain reaction. The
result showed that HPV prevalence was 16.89% (25/148) in OSCC and 0% (0/6) in
no-SIL group. 56/148 of OSCC (37.84%) were p16INK4a positive and the patterns of
p16INK4a expression were characterized to grade 1 (9 cases), grade 2 (15 cases) and
grade 3 (32 cases). 1/6 of no-SIL (16.67%) was p16INK4a positive and classified as
grade 3. p16INK4a expression in OSCC was found in 48% of HPV positive cases and
35.77% of HPV negative cases. No association between p16INK4a expression and
HPV infection in OSCC. This result demonstrated that p16INK4a expression in OSCC
was found in different patterns and not limited to HPV infection and suggested that
p16INK4a expression may not be a perfect surrogate marker for HPV status in OSCC. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
0
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Publication
Journal Publication
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