|
|
Publication
|
| Research Title |
MOLECULAR DETECTION OF SCHISTOSOMA MEKONGI IN INFECTED SNAILS AND RAT FAECES USING A REAL-TIME PCR ASSAY WITH FRET HYBRIDISATION PROBES |
| Date of Distribution |
8 February 2012 |
| Conference |
| Title of the Conference |
1st BMSC (Biomrdical Sciences Conference) |
| Organiser |
Faculty of Medical Sciences |
| Conference Place |
Pullman Khon Kaen Raja Orchid Hotel |
| Province/State |
Khon kaen, Thailand |
| Conference Date |
8 February 2012 |
| To |
10 February 2012 |
| Proceeding Paper |
| Volume |
1 |
| Issue |
1 |
| Page |
PS1-15 |
| Editors/edition/publisher |
Associate Professor Dr. Chanvit Leelayuwat |
| Abstract |
Introduction: Schistosomiasis is a tropical parasitic disease caused by blood-dwelling
fluke worms of the genus Schistosoma whose infective stages, the cercariae, are amplified through
mollusks acting as intermediate hosts. People are infected when exposed to fresh water containing
cercariae that penetrate the skin. There are however considerable differences in intensity of infection and
morbidity, depending on the pattern of exposure and the infective species. In Asia, 3 Schistosoma species
cause human infection: Schistosoma japonicum, Schistosoma malayensis, and Schistosoma mekongi. S.
mekongi, a blood-dwelling fluke is a water-borne parasite that found in communities along the lower
Mekong river basin i.e. Cambodia and Lao PDR.
Methods: This study was developed a real-time PCR assay combined with melting curve
analysis to detect S. mekongi in experimentally infected snails and in fecal samples of infected rats. The
procedure is based on melting curve analysis of a hybrid between an amplicon from S. mekongi
mitochondrion sequence, the 260 bp sequence specific to S. mekongi, and specific fluorophore-labeled
probes.
Results: All S. mekongi-infected snails and from infected rat fecal samples were positive.
Non-infected snails, non-infected rat feces, and genomic DNA of other control parasites were negative. The
sensitivity and specificity were 100%.
Conclusion: The method could be applied as a fast, reliable, sensitive and specific tool for
cercarial location in water reservoirs in at-risk areas, for epidemiological surveys, and for human
coprodiagnosis in Asian populations as well as in travelers returning from endemic areas. |
| Author |
|
| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
0
|
|
|
|
|
|
|
|
|
Journal Publication
ไทย