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Publication
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| Title of Article |
Development of a plant regeneration system from seed-derived shoot segments of rice (Oryza sativa L.) |
| Date of Acceptance |
16 July 2014 |
| Journal |
| Title of Journal |
Asian Journal of Crop Science |
| Standard |
SCOPUS |
| Institute of Journal |
Asian Network for Scientific Information |
| ISBN/ISSN |
1994-7879 |
| Volume |
6 |
| Issue |
4 |
| Month |
|
| Year of Publication |
2014 |
| Page |
305-319 |
| Abstract |
This study was carried out to establish a simple, efficient and reproducible protocol for plant regeneration in four rice genotypes, which are Pokkali, RD6, Sakon Nakhon and Niaw Ubon 2. Two protocols were proposed and compared in this study, including (i) multiple shoots induction and rooting, and (ii) callus induction and regeneration. Single seeds of each cultivar were sown, and germinated seed and shoot segments were used as explant source for the experiments. The results showed that a great number of multiple shoots induced from germinated seed could be achieved on MS solid medium supplemented with 50 µM 6-BAP alone, whereas rooting of the obtained shoots was easily induced on hormone-free MS solid medium. Compact callus formation could be induced from shoot segments on MS solid medium amended with 2 mg/L 2,4-D alone, whilst a combination of 0.5 mg/L NAA and 2.0 mg/L 6-BAP was most effective in promoting shoot regeneration from compact calli. Our study suggests that the regeneration frequency obtained via callus induction was inferior to that yielded through multiple shoots induction. The procedure reported here is expected to be useful in micropropagation, and in vitro breeding and selection for drought and/or salt tolerance in rice, for increasing the yield potential of this vital crop. |
| Keyword |
Oryza sativa, callus induction, direct shoot induction, indica rice, single seeds |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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