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Publication
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| Research Title |
Ultrasound-assisted surfactant-enhanced emulsification microextraction coupled with high performance liquid chromatography for the determination of benzimidazole anthelmintics |
| Date of Distribution |
8 January 2014 |
| Conference |
| Title of the Conference |
Pure and Applied Chemistry International Conference 2014 (PACCON2014) |
| Organiser |
Department of Chemistry, Faculty of Science, Khon Kaen University and Chemical Society of Thailand under the Patronage of Her Royal Highness Princess Chulabhorn Mahidol |
| Conference Place |
Centara Hotel and Convention Centre, Khon Kaen, Thailand |
| Province/State |
ขอนแก่น |
| Conference Date |
8 January 2014 |
| To |
10 January 2014 |
| Proceeding Paper |
| Volume |
2014 |
| Issue |
ANC P033 |
| Page |
111 |
| Editors/edition/publisher |
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| Abstract |
A simple ultrasound-assisted surfactant-enhanced emulsification microextraction (UASEME) coupled with high performance liquid chromatography (HPLC) has been developed for extraction and determination of six benzimidazole anthelmintics, including oxfendazole, albendazole, mebendazole, flubendazole, fenbendazole and niclosamide. The separation was achieved within 12 min, using an Inertsil®ODS column (4.6 x 150 mm, 5.0 µm), with a gradient mobile phase of acetonitrile and 0.1% (v/v) formic acid. Parameters affecting the extraction efficiency, such as kind and volume of the extraction solvent, type and concentration of the surfactant, ultrasound extraction time, centrifugation time and salt addition were investigated. Under the optimum conditions using Tergitol TMN-6 and 1-octanol as emulsifier and extraction solvent, respectively, linearity was in the range of 0.05–7.0 mg L−1 with the correlation coefficients (r2) ranging from 0.991 to 0.997. Enrichment factors were obtained up to 64, corresponding to limits of detection ranging from 0.001–0.007 mg L−1. Intra-day (n=8) and inter-day (n=3x4) precisions were obtained with relative standard deviations for retention time and peak area of lower than 1% and 12%, respectively. The proposed method was successfully applied to determine the target benzimidazoles in milk formulae. |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย