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Publication
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| Title of Article |
Optimization study of suitable pre-reduction agents for selenium analysis in tomato samples by flow injection-hydride generation atomic absorption spectrometry |
| Date of Acceptance |
26 December 2014 |
| Journal |
| Title of Journal |
International Food Research Journal |
| Standard |
SCOPUS |
| Institute of Journal |
Faculty of Food Science and Technology Universiti Putra Malaysia |
| ISBN/ISSN |
22317546 |
| Volume |
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| Issue |
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| Month |
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| Year of Publication |
2015 |
| Page |
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| Abstract |
The pre-reducing agents for Se speciation analysis were subject to optimize under the on-line reduction conditions of flow injection-hydride generation atomic absorption spectrometry using 2 M HCl as a carrier solution and 0.3%(w/v) NaBH4 in 0.2%(w/v) NaOH as a reducing agent. For total Se, the pre-reduction of Se6+ to Se4+ must be completed when optimizing the pre-reducing conditions using (i) conc. HCl, (ii) 5%(w/v) KBr/conc. HCl, (iii) 15%(w/v) KBr/dilute HCl and (iv) 10%(w/v) thiourea/conc. HCl, under heating for 30 min to overcome their matrix effects on the calibration curve using either Se4+ or Se6+ as standard compound. From the results, all of these pre-reducing agents, except that in the presence of thiourea interfered only the calibration curve using Se4+, could be used with both Se species as expressed in terms of their conversion factors of 98-116% and 81-103% for Se4+ and Se6+, respectively. The linear range was obtained between 5-25 µg/L with 0.5 µg/L LOD and 2 µg/L LOQ. The optimal pre-reducing agent was applied for real tomato varieties, resulting in almost satisfactory recoveries (75.6-115.9%). The Se contents in these samples were found to be 0.07-0.10 µg/g, 0.43-0.92 µg/g and 0.50-0.99 µg/g for Se4+, Se6+ and total Se, respectively. |
| Keyword |
Selenium, Speciation, Pre-reduction, Tomato, FI-HGAAS |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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