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Publication
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| Title of Article |
Performance of vancomycin and teicoplanin disk diffusion test in isogenic vancomycin non-susceptible Staphylococcus aureus |
| Date of Acceptance |
9 January 2015 |
| Journal |
| Title of Journal |
Journal of Infection in Developing Countries |
| Standard |
ISI |
| Institute of Journal |
J INFECTION DEVELOPING COUNTRIES,JIDC CENT OFF PORTO CONTE RICERCHE RES CTR, S P 55, PORTO CONTE CAPO CACCIA KM 8.400 LOC, TRAMANIGLIO 07041, ITALY |
| ISBN/ISSN |
1972-2680 |
| Volume |
2015 |
| Issue |
2 |
| Month |
February |
| Year of Publication |
2015 |
| Page |
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| Abstract |
Introduction: Detection of heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) is currently problematic and still pending. Although the population analysis profile with area under the curve (PAP-AUC) is the golden standard for detecting hVISA strains, this method is time-consuming. This study was to induce vancomycin non-susceptible Staphylococcus aureus isolates in methicillin-resistant S. aureus (MRSA) and determine the performance of vancomycin and teicoplanin disk diffusion test for screening of the induced and natural vancomycin non-susceptible isolates.
Methodology: Vancomycin resistance was induced in vitro in methicillin-resistant S. aureus by serial passage in media with increasing vancomycin concentrations. All test isolates were confirmed for their susceptibility to vancomycin by using a PAP-AUC method. The performance of vancomycin and teicoplanin disk diffusion test for detecting both induced and natural hVISA/VISA isolates was analysed using the MedCal program version 10.2.0.
Results: The induction test revealed that 42 of 78 MRSA isolates (53.8%) became hVISA and/or VISA. Using 10, 15, 20, 30 µg vancomycin disks and 30 µg teicoplanin disk, the highest performance (88.9%) for hVISA/VISA detection (71.1%, sensitivity, 100% specificity, 100% PPV and 75% NPV) was obtained when 20 µg vancomycin disk was used at 1.0 McFarland inoculum for 24-hr incubation.
Conclusions: The result indicated that using 20 µg vancomycin disk and bacterial inoculum of 1.0 McFarland is simple to perform and enable to obtain a primary result for hVISA/VISA screening within 24 hours.
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| Keyword |
Staphylococcus aureus; vancomycin resistance; teicoplanin; induction; PAP-AUC |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Attach file |
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| Citation |
0
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Journal Publication
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