| Research Title |
Burkholderia pseudomallei BIOFILM FORMATION FACILITATE BACTERIAL ADHESION TO HUMAN LUNG EPITHELIAL CELLS |
| Date of Distribution |
3 December 2014 |
| Conference |
| Title of the Conference |
The 40 th Congress on Science and Technology of Thailand (STT40) " Science and Technology towards ASEAN Development " |
| Organiser |
The Science Society of Thailand under the Patronage of His Majesty the King in Association with Faculty of Science Khon Kaen University |
| Conference Place |
Pullman Khon Kaen Raja Orchid Khon Kaen, Thailand |
| Province/State |
Khon Kaen |
| Conference Date |
2 December 2014 |
| To |
4 December 2014 |
| Proceeding Paper |
| Volume |
40 |
| Issue |
H-H0051 |
| Page |
265 |
| Editors/edition/publisher |
|
| Abstract |
Melioidosis is classically characterized by pneumonia and multiple abscesses,
with a mortality rate of up to 40%. This tropical disease caused by ingestion, percutaneous
inoculation or inhalation of the Gram-negative soil saprophyte Burkholderia pseudomallei.
The biofilm formation of the B. pseudomallei is influenced by environmental factors and
may enhance the pathogenesis of the bacteria. To investigate the role of B. pseudomallei
biofilm during pathogenesis, B. pseudomallei H777 (biofilm wild type) and M10 (biofilm
mutant) were co-cultured with the human lung epithelial cells (A549) at MOI 10 at 37 o C for
1, 4, 8 and 12 h to determine the role of biofilm in bacterial adhesion, internalization and
intracellular multiplication using antibiotic protection assay. The B. pseudomallei biofilm
mutant demonstrated a significant impairment of A549 cell adhesion compared to the wild
type (p < 0.05). These results suggest that the B. pseudomallei biofilm play an important role
during the first step of bacterial pathogenesis to adhere to the host cells. However, the
biofilm did not show any responsibility during bacterial internalization or intracellular
multiplication in human lung epithelial cells (p > 0.05). (abstract only) |
| Author |
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| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
ไทย