| Research Title |
EFFECT OF NANOENCAPSULATED CURCUMIN ON THE RESOLUTION OF PERIDUCTAL FIBROSIS AND IMPROVES BILE CANALICULI ALTERATION IN OPISTHORCHIS VIVERRINI-INFECTED HAMSTER AFTER PRAZIQUANTEL TREATMENT |
| Date of Distribution |
18 July 2014 |
| Conference |
| Title of the Conference |
The Second Symposium of Research Cluster: Specific Health Problems in Greater Mekong Sub-region (SHeP-GMS) The National Research University Project |
| Organiser |
Specific Health Problems in Greater Mekong Sub-region (SHeP-GMS) The National Research University Project |
| Conference Place |
คณะแพทยศาสตร์ มหาวิทยาลัยขอนแก่น |
| Province/State |
ขอนแก่น |
| Conference Date |
18 July 2015 |
| To |
18 July 2015 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
- |
| Editors/edition/publisher |
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| Abstract |
Introduction: Opisthorchis viverrini infection induces inflammation, bile canaliculi (BC) changes and liver injury leading to increase periductal fibrosis with time and increase a risk for cholangiocarcinoma development. Although praziquantel (PZQ) treatment would be valuable for O. viverrini elimination; however, periductal fibrosis usually remains for a long time after treatment at chronic infection.
Methods: We developed the nanoencapsulated curcumin and evaluated activity against periductal fibrosis and BC alterations in O. viverrini-infected hamsters and PZQ treatment. Sixty hamsters were divided into 5 groups: (1) normal hamsters, (2) O. viverrini-infected hamsters (OV), (3) OV treated with praziquantel (OP) and supplemented with diluent of nanoencapsulated curcumin (OPD), (4 and 5) OP and supplemented with curcumin at 50 mg/kg BW (OPC), and nanoencapsulated curcumin at 50 mg/kg BW (OPN), respectively. Each group was divided into two subgroup, acute (1 month) and chronic (3 months) post-infection. Nanoencapsulated curcumin and curcumin were orally administered daily for one month after PZQ-treatment.
Results: The histopathological studies evaluated by picrosirius red staining showed that the most thickness of periductal fibrosis was found in OV and OPD groups. Compared to OPD group, the thickness of periductal fibrosis was significantly reduced by nanoencapsulated curcumin rather than curcumin treatment. This result was reflected by immunohistochemical staining of α-SMA, fibronectin, vimentin and CTGF expression. Reduction of the mRNA expression of fibrotic markers (MMP-2, -9, TIMP-3, CTGF, α-SMA, fibronectin, collagen I and TGF-β) and decrease of plasma hydroxyproline was also supported the histological finding. Moreover, bile canaliculi feature revealed that increase expression of neprilysin (CD10) and multidrug resistance-associated protein 2 (Mrp2) at the transcription and translation levels were recovered by nanoencapsulated curcumin treatment rather than curcumin supplement.
Conclusions: Nanoencapsulated curcumin shows high efficacy than curcumin to reduce periductal fibrosis and improve bile calicular structure after praziquantel treatment in opisthorchiasis which may be used as a chemopreventive agent to reduce risk of cholangiocarcinoma.
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| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
true |
| Award Title |
The BEST Poster presentation |
| Type of award |
รางวัลด้านวิชาการ วิชาชีพ |
| Organiser |
Specific Health Problems in Greater Mekong Sub-region (SHeP-GMS) The National Research University Project |
| Date of awarding |
18 กรกฎาคม 2558 |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
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