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Publication
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| Research Title |
Reduction of chronic periductal fibrosis in Opisthorchis viverrini-infected hamsters by nanoencapsulated curcumin and praziquantel treatment |
| Date of Distribution |
18 May 2015 |
| Conference |
| Title of the Conference |
International Congress of Liver Flukes and Cholangiocarcinoma:Towards Control and Elimination |
| Organiser |
Liver Fluke and CCA research Center, CASCAP, Department of Parasitology |
| Conference Place |
Pullman Raja Orchid Hotel |
| Province/State |
KhonKean |
| Conference Date |
11 May 2015 |
| To |
12 May 2015 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
- |
| Editors/edition/publisher |
- |
| Abstract |
The thickness of periductal fibrosis induced by chronic Opisthorchis viverrini infection remains for long time after worm elimination and might possible risk to cholangiocarcinoma (CCA) development. We aimed to investigate the effect of nanoencapsulated curcumin (NEC) on a reduction of periductal fibrosis induced by O. viverrini infection and treatment with praziquantel (PZQ). Seventy two hamsters were divided into 2 groups: acute and chronic infection and each experimental group was divided into six sub-groups: (1) normal control (N); (2) O. viverrini-infected (OV); (3) OV group and treated with PZQ (OP); (4) OP group and treated with sphere (diluent of NEC) (OP+Sphere); (5) OP group and treated with curcumin 50 mg/kg BW (OP+Cur); (6) OP group and treated with NEC 50 mg/kg BW (OP+NEC). O. viverrini-infected hamsters were treated with PZQ at 1 and 3 months post-infections for acute and chronic infection groups and each group were orally administered daily with sphere, NEC or curcumin for 1 month. In acute infection, the thickness of periductal fibrosis, mRNA expression of matrix metalloproteinases (MMPs) -2, 7, 9 and 13, tissue inhibitors of MMPs (TIMPs) -1, 2 and 3, collagen I and III, cytokines (TGF-β and TNF-α) were significantly decreased in similar levels in OP, OP+Cur and OP+NEC groups compared to OV group . In chronic infection, periductal fibrosis was significantly reduced in OP+NEC group while fibrotic lesion was persistent in OP or OP+Cur groups. The highest activity of NEC (OP+NEC group) to resolution of fibrosis was supported by the transcriptional and translational expressions of fibrotic markers (α-SMA, CTGF, fibronectin, collagen I, collagen III) and hydroxyproline content in the serum. Moreover, a reduction of the fibrotic makers (α-SMA, fibronectin, CTGF and vimentin) expression were confirmed by NEC-treated primary peribiliary myofibroblasts cells in in vitro study. In conclusion, NEC enhances periductal fibrosis resolution in chronic opisthorchiasis after PZQ treatment which is a promising chemopreventive agent to reduce risk of CCA. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย