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Publication
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| Research Title |
Quantitative proteomic analysis for the identification of candidate biomarkers in cholangiocarinoma in bile |
| Date of Distribution |
16 June 2015 |
| Conference |
| Title of the Conference |
RGJ-Ph.D. Congress XVI"ASEAN: Emerging Research Opportunities" |
| Organiser |
The Royal Golden Jubilee Ph.D Program, The Thailand Research Fund |
| Conference Place |
Jomtien Palm Beach Hotel&Resort |
| Province/State |
Pattaya, Chonburi |
| Conference Date |
16 June 2015 |
| To |
16 June 2015 |
| Proceeding Paper |
| Volume |
1 |
| Issue |
- |
| Page |
226 |
| Editors/edition/publisher |
RGJ-PHD network |
| Abstract |
Introduction and Objective
Cholangiocarcinoma (CCA), a primary malignant tumor, arises from biliary tract epithelia either within the liver or the biliary tract. CCA is a major public health problem in the Northeast of Thailand with the highest incidence and mortality rate in the world. Because it lacks early symptoms and there are a range of possible alternative diagnoses, patients with CCA usually present at an advanced stage, beyond successful treatment. The CCA intact cell and its products can be exfoliated and pass through the bile duct and be found in the bile. Consequently, novel cancer biomarkers might be discovered in the bile and be detected in the feces after it has passed through the gastrointestinal tract.
Methods
In our study 8 bile cases of 2 Mass forming types, 2 Periductal infiltrating types, 2 Intraductal growth types and 2 non-cancer controls were labeled with Isobaric tags for relative and absolute quantitation (iTRAQ®) to determine the relative abundance of bile proteins identified by liquid chromatography-mass spectrometry (LC-MS/MS). The results were validated with label-free quantitative acquisition, sequential window acquisition of all theoretical fragment-ion spectra (SWATH®) and immunohistochemical study in 304 cases of CCA tissue microarray (TMA) sections in 2 candidate proteins.
Results
A total of 1240 unique proteins were identified and eighty-five proteins were significantly elevated in CCA compared to control group, including several candidate CCA biomarkers: clusterin, hemopexin, alpha-1-antitrypsin, ICAM-1, myeloblastin, osteopontin, cystatin-c, and vitronectin. In 305 cases of immunohistochemical study, osteopontin and alpha-1-antitrypsin were significantly related to gross type classification and the alpha-1-antitrypsin was significantly related to cancer stage.
Conclusion
Many up-regulated proteins in CCA bile have been comfirmly found in CCA tissues, therefore CCA intact cell and its products can be exfoliated and pass through the bile duct and be found in the bile. The gross type structure and stage of CCA must be related with CCA exfoliation or bile flow. Validating CCA products in feces with antibody-based techniques is being planned.
Keywords: cancer biomarker, cholangiocarcinoma, proteomics, quantitative proteomics
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| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
true |
| Award Title |
outstanding oral presentation |
| Type of award |
รางวัลด้านวิชาการ วิชาชีพ |
| Organiser |
The Royal Golden Jubilee Ph.D Program, The Thailand Research Fund |
| Date of awarding |
13 มิถุนายน 2558 |
| Attach file |
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| Citation |
2
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Journal Publication
ไทย