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Publication
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| Title of Article |
Biodegradation of microcystin [Dha7]MC-LR by a novel microcystin-degrading bacterium in an
internal airlift loop bioreactor |
| Date of Acceptance |
3 September 2015 |
| Journal |
| Title of Journal |
Water Science and Technology |
| Standard |
SCOPUS |
| Institute of Journal |
IWA |
| ISBN/ISSN |
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| Volume |
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| Issue |
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| Month |
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| Year of Publication |
2015 |
| Page |
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| Abstract |
Cyanobacterial hepatotoxin microcystins are a health problem found in bodies of
water in Thailand. Bacteria capable of biodegrading microcystin Dha7MC-LR were isolated
from the Bueng Nong Khot reservoir in Khon Kaen, Thailand. The bacterium
Novosphingobium isolate KKU25s was shown to degrade Dha7MC-LR at a concentration of
25 µg l-1 at 30°C within 24 h. Two intermediate by-products (linearized peptide and
tetrapeptide) and a cluster gene (mlrA, mlrB, mlrC and mlrD) that encodes four putative
enzymes involved in Dha7MC-LR degradation were detected in KKU25s. KKU25s was
also shown to form strong biofilms in microtiter plate assays. These assays were carried out
in preparation for use of the bacterium in a bioreactor for Dha7MC-LR degradation. In an
internal airlift loop bioreactor (IAL bioreactor), the biodegradation of Dha7MC-LR by the
bacterium was established in batch and continuous flow experiments. In the batch
experiment, KKU25s degraded Dha7MC-LR at a concentration of 25 µg l-1 at 30°C within
24 h, whereas in the continuous flow experiment, KKU25s degraded the toxin at the same
concentration within 36 h. This study demonstrated that this bacterium could potentially be
used to remove microcystins from water. |
| Keyword |
Biodegradation, Microcystin, Novosphingobium, mlr gene, By-product, Bioreactor |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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