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Publication
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| Research Title |
The association between GPX3 gene polymorphism (rs1946234) and diabetes mellitus in Thais |
| Date of Distribution |
11 December 2014 |
| Conference |
| Title of the Conference |
Pan-Asian Biomedical Sciences Conference |
| Organiser |
the ASIA-International Biomedical Science Consortium |
| Conference Place |
Hong Kong Science Park, Shatin, Hong Kong Special Administrative Region |
| Province/State |
Shatin, Hong Kong |
| Conference Date |
11 December 2014 |
| To |
12 December 2014 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
- |
| Editors/edition/publisher |
- |
| Abstract |
Introduction: Diabetes mellitus (DM) is a major risk factor for cardiovascular diseases (CVD). It is characterized by chronic hyperglycemia resulting from defects in insulin action (insulin resistance) and insulin secretion. The prevalence of DM in Thai population is 280 cases per 100,000 individuals. Oxidative stress plays major role in the pathogenesis of DM by induction of -cell dysfunction and insulin resistance. Glutathione peroxidase-3 (GPX3) is one of the prominent antioxidant enzymes involving in the detoxification of hydrogen peroxide and lipid hydro-peroxides, the causes of insulin resistance. The GPX3 mRNA is found in kidney, lung heart, liver, and adipose tissue. Genetic polymorphism within GPX3 promoter region (rs1946234; -1006A>C) has been reported to alter the expression of GPX3 that may implicate in insulin resistance. However, the association of GPX3 gene polymorphism with DM has remained undetermined. The aim of this study was therefore to investigate the association between GPX3 gene polymorphism (rs1946234) and DM in Thais.
Method: A total of 397 community-based Thai subjects (average age ± SD: 51.6±10.1 years; 242 males and 155 females) were recruited, divided into 376 non-DM and 21 DM individuals, and determined for biological parameters. GPX3 gene polymorphism (rs1946234) was determined in genomic DNA using allele specific polymerase chain reaction (AS-PCR) technique.
Result: The respective frequencies of A and C alleles in studied subjects were 85.0% and 15.0%. As compared with AA genotype, C allele carriers had significantly higher fasting blood sugar (FBS) level (p=0.035), but presented with significantly lower low-density lipoprotein cholesterol (LDL-C) level (p=0.034). There were no differences for other lipid parameters, serum high-sensitive C-reactive protein (hs-CRP), and malondialdehyde (MDA) levels among different genotype groups. By comparing between non-DM and DM individuals, the C allele carriers were found to associate with the increase risk of DM with OR = 3.08 (95% CI 1.27-7.47). However, despite showing association with LDL-C level, this polymorphism presented no association with dyslipidemia.
Conclusion: This study demonstrated the association of GPX3 genetic variation, rs1946234, with the increase risk of DM. This might result from the lower level of GPX3 expression and oxidative protection in C carriers.
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย