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Publication
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| Research Title |
The effect of PON1 Gln192Arg polymorphism (rs662) on oxidative stress in Thai females |
| Date of Distribution |
16 July 2014 |
| Conference |
| Title of the Conference |
the International Medical Sciences Conference 2014 |
| Organiser |
The Faculty of Associated Medical Sciences, Khon Kaen University |
| Conference Place |
the Pullman Khon Kaen Raja Orchid Hotel in Khon Kaen, Thailand |
| Province/State |
Khon Kaen, Thailand |
| Conference Date |
15 July 2014 |
| To |
17 July 2014 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
- |
| Editors/edition/publisher |
- |
| Abstract |
Introduction: Oxidative stress contributes to the pathogenesis of cardiovascular diseases (CVD), diabetes, and aging. It causes oxidative-damage to protein, lipid, and DNA. Lipid peroxidation resulting from free radical-induced oxidation can lead to the destruction of cell/organellar membranes and lipoproteins. Paraoxonase 1(PON1) is a high density lipoprotein (HDL)-associated antioxidant enzyme. It protects cell membrane and lipoproteins from oxidative damage. Studies have suggested that estrogen prevents women from CVD. Estrogen also induces anti-oxidative activity of PON1. Despite, there is a high incidence of CVD in Asian women which may involve genetic-associated risk factors. Genetic polymorphism within PON1 that changes amino acid 192 (Gln192Arg) alters the enzymetic protection on low-density lipoprotein (LDL) from oxidation; the Gln form is the most protective, whereas Arg is associated with a higher risk of CVD. The aim of present study was to investigate the effect of PON1-Gln192Arg polymorphism on lipid peroxidation in Thai females.
Method: A total of 138 Thai females, never-smokers, normal function tests for liver and kidney, were recruited. The PON1-Gln192Arg polymorphism was determined using allele-specific polymerase chain reaction (AS-PCR) technique. Blood level of malondialdehyde (MDA) as lipid-peroxidation marker was measured by thiobarbituric acid method.
Result: The respective genotype frequencies in studied subjects for Gln/Gln, Gln/Arg, and Arg/Arg were 10.9%, 44.2%, and 44.9%, with allele frequencies of Gln and Arg were 33.0% and 67.0%. As compared with Gln/Gln genotype, Arg/Arg group had significantly higher blood MDA level (p=0.022). There were no differences for lipid profile, serum high-sensitive C-reactive protein (hs-CRP) level, and white blood cell count among different genotype groups.
Conclusion: This study demonstrated the effect of PON1 genetic variations on lipid peroxidation by which Arg/Arg genotype have less protective effect than Gln/Gln. The PON1 Arg/Arg may therefore increase the risk of oxidative stress-related diseases in Thai women, at least in the present study. |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย