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Publication
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| Title of Article |
Drug susceptibility and biofilm formation of Burkholderia
pseudomallei in nutrient-limited condition |
| Date of Acceptance |
4 October 2014 |
| Journal |
| Title of Journal |
Tropical Biomedicine |
| Standard |
ISI |
| Institute of Journal |
THE MALAYSIAN SOCIETY OF PARASITOLOGY AND TROPICAL MEDICINE |
| ISBN/ISSN |
ISSN 01239-5720 |
| Volume |
32 |
| Issue |
2 |
| Month |
June |
| Year of Publication |
2015 |
| Page |
300-309 |
| Abstract |
Burkholderia pseudomallei is the causative agent of melioidosis, which can form
biofilms and microcolonies in vivo and in vitro. One of the hallmark characteristics of the
biofilm-forming bacteria is that they can be up to 1,000 times more resistant to antibiotics
than their free-living counterpart. Bacteria also become highly tolerant to antibiotics when
nutrients are limited. One of the most important causes of starvation induced tolerance in
vivo is biofilm growth. However, the effect of nutritional stress on biofilm formation and drug
tolerance of B. pseudomallei has never been reported. Therefore, this study aims to determine
the effect of nutrient-limited and enriched conditions on drug susceptibility of B. pseudomallei
in both planktonic and biofilm forms in vitro using broth microdilution method and Calgary
biofilm device, respectively. The biofilm formation of B. pseudomallei in nutrient-limited and
enriched conditions was also evaluated by a modified microtiter-plate test. Six isolates of
ceftazidime (CAZ)-susceptible and four isolates of CAZ-resistant B. pseudomallei were used.
The results showed that the minimum bactericidal concentrations of CAZ against B.
pseudomallei in nutrient-limited condition were higher than those in enriched condition. The
drug susceptibilities of B. pseudomallei biofilm in both enriched and nutrient-limited conditions
were more tolerant than those of planktonic cells. Moreover, the quantification of biofilm
formation by B. pseudomallei in nutrient-limited condition was significantly higher than that
in enriched condition. These data indicate that nutrient-limited condition could induce biofilm
formation and drug tolerance of B. pseudomallei.
INTRODUCTION
Burkholderia pseudomallei is recognized
as an emerging pathogen, responsible for
melioidosis. Infections have been
increasingly reported in many countries
in tropical and subtropical regions of the
world (Currie et al., 2008; Rolim et al., 2009;
Mukhopadhyay et al., 2011). Burkholderia
pseudomallei is an intrinsically resistant to
many of the currently marketed antibiotics,
including third-generation cephalosporins,
penicillins, rifamycins, aminoglycosides
(White, 2003; Cheng & Currie, 2005),
quinolones and macrolides which limit
therapeutic |
| Keyword |
Burkholderia pseudomallei, antibiotic tolerance, drug susceptibility, biofilm formation, nutrient-limited, melioidosis |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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