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Publication
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| Title of Article |
Animal Species Identification through High Resolution Melting Real time PCR (HRM) of the 1 Mitochondrial 16S rRNA Gene |
| Date of Acceptance |
27 October 2015 |
| Journal |
| Title of Journal |
Annals of Animal Science |
| Standard |
ISI |
| Institute of Journal |
DE GRUYTER |
| ISBN/ISSN |
2300-8733 |
| Volume |
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| Issue |
in press |
| Month |
12 |
| Year of Publication |
2015 |
| Page |
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| Abstract |
Animal species identification has received growing attention, regarding genetic 19 diversity and food traceability. The objective of this study is to apply a universal primer of 20 part of the mitochondrial 16S rRNA gene analysis using the PCR-RFLP and HRM methods 21 for identification of species origin in cattle, chicken, horse, sheep, pig, buffalo, and goat. PCR 22 product size was 512bp. The PCR product of 16S rRNA was digested with two restriction 23 enzymes (BclI and MseI); sufficient to easily generate analyzable species-specific restriction 24 profiles that could distinguish the unambiguity of all targeted species. The HRM method 25
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successfully identified all species by shape of meting temperature, and proved to be of higher 26 resolution, and a more cost effective, alternative method compared with other identification 27 techniques. |
| Keyword |
16S rRNAgene, HRM, PCR-RFLP, Species Identification |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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