2009-2015 ©
Journal Publication
Title of Article Animal Species Identification through High Resolution Melting Real time PCR (HRM) of the 1 Mitochondrial 16S rRNA Gene 
Date of Acceptance 27 October 2015 
     Title of Journal Annals of Animal Science 
     Standard ISI 
     Institute of Journal DE GRUYTER 
     ISBN/ISSN 2300-8733 
     Issue in press 
     Month 12
     Year of Publication 2015 
     Abstract Animal species identification has received growing attention, regarding genetic 19 diversity and food traceability. The objective of this study is to apply a universal primer of 20 part of the mitochondrial 16S rRNA gene analysis using the PCR-RFLP and HRM methods 21 for identification of species origin in cattle, chicken, horse, sheep, pig, buffalo, and goat. PCR 22 product size was 512bp. The PCR product of 16S rRNA was digested with two restriction 23 enzymes (BclI and MseI); sufficient to easily generate analyzable species-specific restriction 24 profiles that could distinguish the unambiguity of all targeted species. The HRM method 25 2 successfully identified all species by shape of meting temperature, and proved to be of higher 26 resolution, and a more cost effective, alternative method compared with other identification 27 techniques. 
     Keyword 16S rRNAgene, HRM, PCR-RFLP, Species Identification 
547030012-3 Miss PITCHAYANIPA KLOMTONG [Main Author]
Agriculture Doctoral Degree

Reviewing Status มีผู้ประเมินอิสระ 
Status ได้รับการตอบรับให้ตีพิมพ์ 
Level of Publication นานาชาติ 
citation false 
Part of thesis true 
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