| Research Title |
Cellulase enzyme production from agricultural waste
by Acinetobacter sp. KKU44 |
| Date of Distribution |
9 May 2014 |
| Conference |
| Title of the Conference |
The 5th KKU InternationalEngineering Conference 2014 (KKU-IENC 2014) |
| Organiser |
Engineering KhonKaen University |
| Conference Place |
Pullman Khon Kaen Raja Orchid Hotel |
| Province/State |
Khon Kaen |
| Conference Date |
27 March 2014 |
| To |
29 March 2014 |
| Proceeding Paper |
| Volume |
5 |
| Issue |
1 |
| Page |
170 |
| Editors/edition/publisher |
Sujin Bureerat |
| Abstract |
Due to a high ethanol demand, the approach for effective ethanol production is important
and has been developed rapidly worldwide. Several agricultural wastes are highly abundant in
celluloses and the effective cellulase enzymes do exist widely among microorganisms. Accordingly,
the cellulose degradation using microbial cellulase to produce a low-cost substrate for ethanol
production has attracted more attention. In this study, the cellulase producing bacterial strain has
been isolated from rich straw and identified by 16S rDNA sequence analysis as Acinetobacter sp.
KKU44. This strain is able to grow and exhibit the cellulase activity. The optimal temperature for
its growth and cellulase production is 37 °C. The optimal temperature of bacterial cellulase activity
is 60 °C. The cellulase enzyme from Acinetobacter sp. KKU44 is heat-tolerant enzyme. The
bacterial culture of 36 h. showed highest cellulase activity at 120 U/mL when grown in LB medium
containing 2% (w/v). The capability of Acinetobacter sp. KKU44 to grow in cellulosic agricultural
wastes as a sole carbon source and exhibiting the high cellulase activity at high temperature
suggested that this strain could be potentially developed further as a cellulose degrading strain for a
production of low-cost substrate used in ethanol production. |
| Author |
|
| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
0
|
|
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Publication
Journal Publication
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