| Research Title |
CIRCULATING CD64/CD163 MONOCYTE RATIO CORRELATING WITH TUMOR M2 MACROPHAGE DENSITY IN HPV ASSOCIATED CERVICAL LESION SEVERITY. |
| Date of Distribution |
21 September 2015 |
| Conference |
| Title of the Conference |
The 30th International Papillomavirus Conference & Clinical Workshop (HPV 2015) |
| Organiser |
International Papillomavirus Society |
| Conference Place |
CCL - Centro de Congressos de Lisboa |
| Province/State |
Lisbon, Portugal |
| Conference Date |
17 September 2015 |
| To |
21 September 2015 |
| Proceeding Paper |
| Volume |
2015 |
| Issue |
1 |
| Page |
HPV15-0274 |
| Editors/edition/publisher |
International Papillomavirus Society |
| Abstract |
Introduction:. Macrophage can differentiate into M1 - or M2-macrophage depending on microenvironment. The M2 suppresses inflammation and supports tumor growth. Infiltrating macrophages were gradually increased corresponding to severity of cervical lesions infected with high-risk human papillomavirus (HR-HPV). Changing of peripheral monocyte
subpopulation according to infiltrating M2 density may predict severity of HPV associated cervical lesions.
Objectives: To investigate circulating monocyte subpopulation, HPV infection and tissue infiltrating M2 in women with normal cytology, no squamous intraepithelial lesion (No-SIL), low-grade SIL (LSIL), high-grade SIL (HSIL) and cervical cancer.
Methods: Heparinized bloods were analyzed for monocyte subpopulation by flow cytometry. Formalin-fixed, paraffin-embedded (FFPE) cervical tissues were used for HPV detection by GP5+/6+ PCR and E6/E7 mRNA in situ hybridization. Tissue-infiltrating CD163+ M2 were determined by immunohistochemistry. CD163 and Arginase I gene expression in fresh cervical tissues were performed by real-time PCR.
Results: Elevation of CD16+ monocytes was found in women with cervical lesions and inflammation. Interestingly, CD64+ monocytes were significantly elevated only in HSIL and cervical cancer groups. Amount of CD163+ and IL10+ monocytes in each group showed no significant difference, however, CD163+ monocytes gradually decreased in higher grades of cervical lesions. Infiltrating CD163+ M2 mainly found in stroma/peritumor areas corresponded to CD163 and Arginase I gene expression and significantly increased (>score II) according to HPV infection and cervical lesion severity. Ratio of circulating CD64+/CD163+ monocytes showed a correlation with the CD163+ M2 infiltration.
Conclusions: The circulating CD64+/CD163+ monocyte ratio may be a predictor of M2 infiltration and HR-HPV associated cervical cancer progression. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
true |
| Award Title |
The HPV 2015 Travel Scholaship award |
| Type of award |
รางวัลด้านวิชาการ วิชาชีพ |
| Organiser |
International Papillomavirus Society |
| Date of awarding |
19 กันยายน 2558 |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
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