2012 ©
             Publication
Journal Publication
Research Title CIRCULATING CD64/CD163 MONOCYTE RATIO CORRELATING WITH TUMOR M2 MACROPHAGE DENSITY IN HPV ASSOCIATED CERVICAL LESION SEVERITY. 
Date of Distribution 21 September 2015 
Conference
     Title of the Conference The 30th International Papillomavirus Conference & Clinical Workshop (HPV 2015) 
     Organiser International Papillomavirus Society 
     Conference Place CCL - Centro de Congressos de Lisboa 
     Province/State Lisbon, Portugal 
     Conference Date 17 September 2015 
     To 21 September 2015 
Proceeding Paper
     Volume 2015 
     Issue
     Page HPV15-0274 
     Editors/edition/publisher International Papillomavirus Society 
     Abstract Introduction:. Macrophage can differentiate into M1 - or M2-macrophage depending on microenvironment. The M2 suppresses inflammation and supports tumor growth. Infiltrating macrophages were gradually increased corresponding to severity of cervical lesions infected with high-risk human papillomavirus (HR-HPV). Changing of peripheral monocyte subpopulation according to infiltrating M2 density may predict severity of HPV associated cervical lesions. Objectives: To investigate circulating monocyte subpopulation, HPV infection and tissue infiltrating M2 in women with normal cytology, no squamous intraepithelial lesion (No-SIL), low-grade SIL (LSIL), high-grade SIL (HSIL) and cervical cancer. Methods: Heparinized bloods were analyzed for monocyte subpopulation by flow cytometry. Formalin-fixed, paraffin-embedded (FFPE) cervical tissues were used for HPV detection by GP5+/6+ PCR and E6/E7 mRNA in situ hybridization. Tissue-infiltrating CD163+ M2 were determined by immunohistochemistry. CD163 and Arginase I gene expression in fresh cervical tissues were performed by real-time PCR. Results: Elevation of CD16+ monocytes was found in women with cervical lesions and inflammation. Interestingly, CD64+ monocytes were significantly elevated only in HSIL and cervical cancer groups. Amount of CD163+ and IL10+ monocytes in each group showed no significant difference, however, CD163+ monocytes gradually decreased in higher grades of cervical lesions. Infiltrating CD163+ M2 mainly found in stroma/peritumor areas corresponded to CD163 and Arginase I gene expression and significantly increased (>score II) according to HPV infection and cervical lesion severity. Ratio of circulating CD64+/CD163+ monocytes showed a correlation with the CD163+ M2 infiltration. Conclusions: The circulating CD64+/CD163+ monocyte ratio may be a predictor of M2 infiltration and HR-HPV associated cervical cancer progression. 
Author
517070025-7 Mr. PIYAWUT SWANGPHON [Main Author]
Medicine Doctoral Degree

Peer Review Status มีผู้ประเมินอิสระ 
Level of Conference นานาชาติ 
Type of Proceeding Abstract 
Type of Presentation Oral 
Part of thesis true 
Presentation awarding true 
     Award Title The HPV 2015 Travel Scholaship award 
     Type of award รางวัลด้านวิชาการ วิชาชีพ 
     Organiser International Papillomavirus Society 
     Date of awarding 19 กันยายน 2558 
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