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Publication
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| Research Title |
Optimization of ethanol production from sugarcane bagasse hydrolysate using isolated thermotolerant yeast Pichia kudriavzevii RZ8-1 |
| Date of Distribution |
5 February 2016 |
| Conference |
| Title of the Conference |
International Conference on Food and Applied Bioscience |
| Organiser |
Faculty of Agro-Industry, Chiang Mai University |
| Conference Place |
The Empress Hotel, Chiang Mai, Thailand |
| Province/State |
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| Conference Date |
4 February 2016 |
| To |
5 February 2016 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
- |
| Editors/edition/publisher |
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| Abstract |
Ethanol production from agro-waste industry provides an alternative energy production system. One of the most suitable cellulosic materials for ethanol production is sugarcane bagasse (SCB), a residue by-product from sugarcane factory. Screenings of thermotolerant yeast capable of producing ethanol from SCB at high temperature were described in this study. SCB was pretreated with two step of 2% (w/v) NaOH followed by 2% (w/v) H2SO4. Enzymatic hydrolysis was conducted using 50 FPU/g DS at 50 0C and pH 5. The sugar concentration obtained after 72 h of enzymatic hydrolysis was 100.70 g/L glucose and 24.80 g/L xylose. Comparative study on ethanol production from SCB hydrolysate by isolated thermotolerant yeasts revealed that P. kudriavzevii RZ8-1 was a good candidate for ethanol production at high temperature. This isolated thermotolerant yeast gave the highest ethanol concentration of 35.51 g/L and 33.84 g/L, productivity of 1.48 and 1.41 g/L.h with the ethanol yield of 0.41 and 0.39 g/l at 37oC and 40oC, respectively. For process optimization, the Placket-Burman design was used to screen the experimental variables and the response surface methodology (RSM) based on the central composite design (CCD) was used to optimize the ethanol fermentation condition. Based on these statistical experimental designs, the optimum conditions for ethanol production from SCB hydrolysate at high temperatures were achieved. At 37oC, the maximum ethanol concentration and productivity of 33.69 g/L and 3.74 g/L.h, respectively, were achieved under the optimum conditions (5x107 cells/mL cell concentration, pH 4.75, and 7.50 g/L yeast extract) after fermentation for 9 h. |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
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