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Publication
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| Title of Article |
Role of RelA and SpoT in Burkholderia pseudomallei survival, biofilm formation and ceftazidime tolerance during nutritional stress |
| Date of Acceptance |
13 May 2016 |
| Journal |
| Title of Journal |
Tropical Biomedicine |
| Standard |
ISI |
| Institute of Journal |
Malaysian Society of Parasitology and Tropical Medicine |
| ISBN/ISSN |
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| Volume |
33 |
| Issue |
4 |
| Month |
December |
| Year of Publication |
2016 |
| Page |
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| Abstract |
Burkholderia pseudomallei is a saprophyte found in soil and stagnant water and is the causative agent of human melioidosis, an often fatal disease. B. pseudomallei is intrinsically resistant to many antibiotics. The stringent response is a global bacterial adaptation process in response to nutritional limitation and is mediated by the alarmone (p)ppGpp, which is produced by two proteins, RelA and SpoT. In order to test whether the stringent response is involved in ceftazidime tolerance, biofilm formation, and bacterial survival in the soil microcosm, B. pseudomallei strain K96243 and its isogenic relA and relAspoT mutants were grown in rich and nutrient-limited media. In nutrient-limiting conditions, both the wild type and mutants were up to 64-times more tolerant to ceftazidime than when grown in rich culture conditions. Moreover, the biofilm formation of all bacterial isolates tested was significantly higher under nutrient-limiting conditions than under nutrient-rich conditions. The relAspoT mutant produced less biofilm than its wild type or relA mutant under nutrient-limiting conditions. The survival of the relAspoT double mutant cultured in 1% moisture content soil was significantly decreased compared to the wild type and the relA mutant. Therefore, the RelA/SpoT protein family might represent a promising target for the development of novel antimicrobial agents to combat B. pseudomallei. |
| Keyword |
Burkholderia pseudomallei, melioidosis, starvation, biofilm, drug tolerance, RelA/SpoT homologue family |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
false |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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