| Research Title |
Anti-biofilms Activity of Peptides against Burkholderia Thailandensis |
| Date of Distribution |
27 March 2015 |
| Conference |
| Title of the Conference |
The 34th National Graduate Research Conference |
| Organiser |
บัณฑิตวิทยาลัย มหาวิทยาลัยขอนแก่น |
| Conference Place |
ห้องประชุมคณะแพทย์ศาสตร์ มหาวิทยาลัยขอนแก่น |
| Province/State |
ขอนแก่น |
| Conference Date |
27 March 2015 |
| To |
27 March 2015 |
| Proceeding Paper |
| Volume |
1 |
| Issue |
1 |
| Page |
530-538 |
| Editors/edition/publisher |
|
| Abstract |
Burkholderia thailandensis is largely avirulent in humans and closely related to Burkholderia pseudomallei, which causes melioidosis. One of the virulent factors of gram-negative bacteria is the ability to produce biofilms. Both of B. pseudomallei and B. thailandensis have been reported to develop biofilms. Therefore, we interest to study meliodosis bacterial biofilms through B.thailandensis as model. Nowaday, Ceftazidem Ceftazidime (CAZ) is used antibiotic currently in melioidosis treatment from B. pseudomallei infection but increasing incidence of CAZ resistance has been reported. Antimicrobial peptides ( AMPs) are alternative novel agents against drug-resistant pathogenic bacteria. Previous study showed that antimicrobial peptides have potential as anti-biofilm agents. In this study, we investigated the activity of CAZ and antimicrobial peptide CA-MA against B.thailandensis UE5. The results showed that 32 μg/mL of CAZ and could not eradicated the biomass of biofilms from B. thailandensis UE5 but can inhibit bacteria inside biofilms dramatically at 128 μg/mL. In contrast, CA-MA (Cecropin-Magainin) at 128 μg/mL can eradicate biomass of biofilms from B. thailandensis UE5 and can inhibit bacteria inside biofilms weakly with the same concentration. FTIR was used to examine and compare changes of the biofilms composition. We found that FTIR fingerprint of biofilms from B.thailandensis UE5 under control condition and CAZ treatment is not different. This indicated that CAZ did not alter biofilms composition. This study has demonstrated the different activity between antimicrobial peptide and antibiotic in inhibiting biofilms. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Full paper |
| Type of Presentation |
Poster |
| Part of thesis |
false |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
ไทย