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Publication
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| Title of Article |
Changes of testicular phosphorylated proteins in
response to restraint stress in male rats |
| Date of Acceptance |
9 November 2015 |
| Journal |
| Title of Journal |
Journal of Zhejiang University-SCIENCE B (Biomedicine & Biotechnology) |
| Standard |
ISI |
| Institute of Journal |
Zhejiang University |
| ISBN/ISSN |
|
| Volume |
17 |
| Issue |
1 |
| Month |
|
| Year of Publication |
2016 |
| Page |
|
| Abstract |
Abstract: Objective: To investigate male reproductive parameters via changes of potential testicular protein markers
in restraint-stress rats. Methods: Male Sprague-Dawley rats were divided into two groups (non-immobilized control and
restraint-immobilized/stress groups, n=8 each group). The stress animals were immobilized (12 h/d) by a restraint
cage for 7 consecutive days. All reproductive parameters, morphology and histology were observed and compared
between groups. In addition, the expression of steroidogenic acute regulatory (StAR) and phosphotyrosine proteins
(previously localized in Sertoli and late spermatid cells) in testicular lysate was assayed by immuno-Western blotting.
Results: Testosterone level, sperm concentration and sperm head normality of stress rats were significantly decreased
while the corticosterone level was increased as compared with the control (P<0.05). Histologically, stress rats showed
low sperm mass in epididymal lumen and some atrophy of seminiferous tubules. Although the expression of testicular
StAR protein was not significantly different between groups, changed patterns of the 131, 95, and 75 kDa testicular
phosphorylated proteins were observed in the stress group compared with the control group. The intensity of a testicular
95-kDa phosphorylated protein was significantly decreased in stress rats. Conclusions: This study has
demonstrated the alteration of testicular phosphorylated protein patterns, associated with adverse male reproductive
parameters in stress rats. It could be an explanation of some infertility in stress males. |
| Keyword |
Restraint-stress rats, Steroidogenic acute regulatory (StAR) protein, Testicular phosphorylated protein |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ตีพิมพ์แล้ว |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Journal Publication
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