| Research Title |
High glucose boosts the metastatic ability of high metastasis cholangiocarcinoma cells via O-GlcNAcylation |
| Date of Distribution |
26 May 2016 |
| Conference |
| Title of the Conference |
The 5th International Biochemistry and Molecular Biology Conference |
| Organiser |
Faculty of Science, Prince of Songkla University |
| Conference Place |
B. P. Samila Beach Hotel |
| Province/State |
Songkla, Thailand |
| Conference Date |
26 May 2016 |
| To |
27 May 2016 |
| Proceeding Paper |
| Volume |
5 |
| Issue |
1 |
| Page |
134 |
| Editors/edition/publisher |
|
| Abstract |
Increased glucose uptake is a key feature of cancer cells and is utilized to produce energy and essential intermediates to support cell survival, proliferation, and metastasis. An association between diabetes millitus and cancer progression has been demonstrated in several cancers including cholangiocarcinoma (CCA), however, the underlying mechanism is not yet clear. Moreover, high glucose induced O-GlcNAcylation that promoted cancer progression has been previously reported. In this study, we aimed to connect the glucose level to the O-GlcNAcylation of proteins and the metastatic potentials in CCA cell lines. The O-GlcNAcylation was regulated by two enzymes; O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) for the addition and a removal of GlcNAc from the O-GlcNAcylated proteins (OGP). UDP-GlcNAc produced from a hexosamine biosynthesis pathway (HBP) also served as a sugar donor for O-GlcNAcylation. The parental and highly metastatic CCA subline were cultured in media with normal glucose (5.6 mM; euglycemic condition) or high glucose (25 mM; hyperglycemic condition). The high glucose condition promoted migration and invasion of both the CCA cells, but the effects were significantly higher in the high metastatic cells. These effects were concomittently observed with the increased global OGP, and the increased expressions of the glycolytic enzymes (hexokinase), HBP enzyme (glucosamine-fructose-6-phosphate amidotransfrase; GFAT) and OGT. A treatment with a GFAT inhibitor - DON, reduced the global OGP level and alleviated CCA cell migration of the high metastatic cells. Altogether, these results indicated that the roles of high glucose in enhancing metastatic potentials of CCA was via modulation of the O-GlcNAcylation reactions. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| Presentation awarding |
true |
| Award Title |
Outstanding Poster Presentation Award |
| Type of award |
รางวัลด้านวิชาการ วิชาชีพ |
| Organiser |
BMB Section, Science Society of Thailand |
| Date of awarding |
27 พฤษภาคม 2559 |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
ไทย