2012 ©
             Publication
Journal Publication
Title of Article Biochemical hydrogen and methane potential of sugarcane syrup using a two-stage anaerobic fermentation process 
Date of Acceptance 3 December 2015 
Journal
     Title of Journal Industrial Crops and Products 
     Standard ISI 
     Institute of Journal Elsvier 
     ISBN/ISSN 0926-6690 
     Volume  
     Issue 82 
     Month
     Year of Publication 2016 
     Page 88-99 
     Abstract The potential of using a two-stage hydrogen and methane fermentation of sugarcane juice was studied.The effects of pure and mixed culture as inocula on hydrogen production were compared. Additionally, the effects of pure culture inocula form, i.e., free cells or immobilized cells of Clostridium butyricum TISTR1032 was compared to different forms of mixed cultures, i.e., granules or suspended cells of heat-treated upflow anaerobic sludge blanket (UASB) granules. The hydrogenogenic effluents from all treatments were used as substrates to evaluate the potential of methane production by non-pretreated UASB granules. Results showed that a pure culture gave a higher hydrogen production potential and a shorter lag time in comparison to mixed cultures. Immobilized cells of C. butyricum TISTR1032 gave a hydrogen production potential that was 1.2 times higher than that of free cells. However, there was no significant difference in hydrogen production potential of granules and suspended cells. Moreover, hydrogenogenic effluent from the first stage fermentation showed a high efficiency in methane production by non-pretreated UASB granules.Although fermentation of mixed cultures resulted in lower hydrogen yield, its hydrogenogenic effluent yielded a higher methane production than that of pure culture. Therefore, overall energy yield when using mixed cultures in hydrogen production stage was higher. The two-stage hydrogen and methane production process removed 94–95% of chemical oxygen demand (COD) resulting in energy recovery in the range of 12–13.4 kJ/gCODadded. Results indicated that the COD removal efficiency and the energy recovery from two-stage hydrogen and methane production was improved 6–7 fold when compared to hydrogen production alone. 
     Keyword Clostridium butyricum, Energy yield, Mixed cultures, Pure culture, UASB granules 
Author
557160005-6 FS 2 CHATCHAWIN NUALSRI [Main Author]
Technology Doctoral Degree

Reviewing Status มีผู้ประเมินอิสระ 
Status ตีพิมพ์แล้ว 
Level of Publication นานาชาติ 
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Part of thesis true 
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