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Publication
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| Research Title |
The relationship between c-myc amplification and human papillomavirus (HPV) infection of oral cancer in Northeast Thailand |
| Date of Distribution |
7 May 2015 |
| Conference |
| Title of the Conference |
Frontier in Cancer Research I: Systems Biology for Cancer Research |
| Organiser |
Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Thailand |
| Conference Place |
Meeting room 3A-C, Wechwichakarn Building, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand |
| Province/State |
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| Conference Date |
7 May 2015 |
| To |
8 May 2015 |
| Proceeding Paper |
| Volume |
- |
| Issue |
- |
| Page |
- |
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| Abstract |
The malignant progression of HPV-infected cells in the oral cavity is likely to participate with alterations at the cellular genome level, commonly occurs through overexpression or activation of several proto-oncogenes. c-myc amplification appears to underlie most of c-Myc overexpression and subsequently moderate cyclin D1 amplification affecting different aspects of cell behaviors. The strong evidence showed that HPV16 infection is associated with c-myc amplification in cervical cancer, whereas in oral cancer is still limited. This study investigated the association of HPV infection with c-myc amplification and theirs correlation with c-Myc expression and cyclin D1 amplification. The extracted DNA from 148 formalin fixed paraffin embedded oral cancer tissue was examined for copy number of c-myc and cyclin D1 gene by real-time PCR (RT-PCR) and normal buccal cells were used as calibrators. c-Myc expression was determined by immunohistochemistry on tissue microarray. HPV infection was investigated by RT-PCR and in situ hybridization and reverse line blot hybridization for HPV genotyping. The relationship between HPV infection and c-myc amplification was analyzed. c-Myc expression, HPV infection and cyclin D1 amplification was statistical analyzed with c-myc amplification as well as histological grade. The result showed that HPV infection was 58.1% and HPV16 was most common found. Interestingly, HPV infection was not statistic significant associated with c-myc amplification (55.4%), whereas associated with cyclin D1 (27%). c-Myc expression was 74.8% and significant associated with c-myc amplification (87.1%). Additionally, c-Myc overexpression was more frequently found in cyclin D1 amplification (76.3%) and HPV infection (76.4%). Only cyclin D1 amplification was significant associated with poorly differentiated squamous cell carcinoma. This result showed that c-myc amplification in oral cancer might be activated by various factors including HPV infection which can promote c-Myc overexpression and cyclin D1 amplification and suggested that in HPV associated oral cancer, degree of oral malignancy might be more aggressive. |
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| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย