| Research Title |
Gene expression profiles during the transient ceftazidime resistance of Burkholderia pseudomallei |
| Date of Distribution |
8 August 2016 |
| Conference |
| Title of the Conference |
8th World Melioidosis Congress 2016 |
| Organiser |
University of Florida Emerging Pathogens Institute and University of Florida Office of the Vice President for Research |
| Conference Place |
Radisson Blu Hotel at Cebu City, The Philippines |
| Province/State |
Cebu City |
| Conference Date |
7 August 2016 |
| To |
10 August 2016 |
| Proceeding Paper |
| Volume |
2016 |
| Issue |
1 |
| Page |
18 |
| Editors/edition/publisher |
|
| Abstract |
Burkholderia pseudomallei is intrinsically resistant to a variety of antibiotics. Ceftazidime, the drug of choice, was reported to fail in treatment even though in vitro culture showed susceptibility. Transient phenotypic changes for survival in response to lethal drug exposure and transient drug resistance investigated during biofilm-induced condition may help explain the phenomenon. The aim of this study is to compare the complete transcriptome profiles of B. pseudomallei grew as planktonic shaking, biofilm forming and planktonic shading. A set of 245 genes (9.9%) was differentially expressed in all conditions. One hundred and fifty-four genes were expressed higher and 91 were lower in a resistance state as compared between biofilm and planktonic shaking. One hundred genes were expressed higher and 145 genes were lower in a sensitive state as compared between planktonic shedding and biofilm form. The total genes that significantly express in each pair were classified according to their function. From the analysis, most of these genes reflected bacterial response to environmental tension. Moreover, genes involving-bacteria morphological change were expressed higher as well. Several genes with unknown functions or hypothetical proteins were also observed that made the interpretation difficult. The system biology algorithm will be used to construct the gene interaction in order to pick-up some interesting genes that linked to transient drug resistant for further study. This search may pave a way to understand how bacteria could survive in human host while the drug of choice is being used. |
| Author |
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| Peer Review Status |
มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Presentation awarding |
false |
| Attach file |
|
| Citation |
0
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Publication
Journal Publication
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