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Publication
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| Research Title |
Exosomes from EBV-Infected Lymphocytes in Cervical Lesions Induced Interferon-Related Gene Expression in Dendritic Cells
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| Date of Distribution |
10 November 2016 |
| Conference |
| Title of the Conference |
2nd ICGEB Workshop on Human Papillomavirus: from Basic Biology to Cancer Prevention |
| Organiser |
The Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong |
| Conference Place |
Kai Chong Tong, G/F, School of Public Health Building, Prince of Wales Hospital, Shatin, HKSAR, China. |
| Province/State |
Hongkong, China |
| Conference Date |
8 November 2016 |
| To |
10 November 2016 |
| Proceeding Paper |
| Volume |
2 |
| Issue |
1 |
| Page |
52 |
| Editors/edition/publisher |
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| Abstract |
Introduction
Epstein-Barr virus (EBV) products released from the infected cells via exosomes can induce biological changes in recipient uninfected cells. Persistent infection of EBV and Human papillomavirus (HPV) has been linked to development of multiple malignancies, however, the role of EBV in HPV-associated cervical cancer is poorly understood. This study aimed to determine the association of EBV infection with HPV-associated cervical carcinogenesis and to investigate the possible contributing role of EBV in development of cervical lesion.
Methods
EBV DNA and HPV DNA were determined in 82 no squamous intraepithelial lesions (noSILs), 85 low-grade SILs (LSILs), 85 high-grade SILs (HSILs) and 40 squamous cell carcinoma (SCC) samples using polymerase chain reaction (PCR). EBV localization was determined by in situ hybridization. EBV-modified exosomes were isolated from supernatant of wild type EBV-infected lymphoblastoid cell lines (LCLs) culture using ultracentrifugation and transferred to recipient cells: HPV16 E6/E7-transduced keratinocytes or monocyte-derived dendritic cells (moDCs). EBER1 and interferon (IFN)-related gene expression in recipient cells were determined by RT-PCR.
Results
EBV DNA was found in 13.4%, 29.4%, 49.4% and 35.0% of noSIL, LSIL, HSIL and SCC, respectively. The prevalence of HPV–EBV co-infections was significantly higher in all grades of lesions than in noSIL samples (p < 0.05), i.e. noSIL (1.2%), LSIL (18.8%), HSIL (41.2%) and SCC (30.0%). EBV was mainly in lymphocytes infiltrating in stroma of cervical lesions. In vitro study demonstrated that EBV-modified exosomes were internalized into recipient cells. EBER1 but not EBER2 was found in recipient cells. Nonetheless, EBER1 from EBV-modified exosomes could not induce IFN-related gene expression in HPV16 E6/E7-transduced keratinocytes. However, expression of some IFN-related genes was increased in moDCs after incubation with EBV-modified exosomes for 24 hours.
Conclusion
EBV-infected lymphocytes infiltrating in cervical lesions may play an indirect role in cervical cancer progression by modulating microenvironment via exosomes and mediator secretion.
Keywords: Human papillomavirus, Epstein-Barr virus, cervical cancer, exosomes
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| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
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