| Title of Article |
A novel probiotic Bacillus siamensis B44v isolated from Thai pickled vegetables (Phak-dong) for potential use as a feed supplement in aquaculture |
| Date of Acceptance |
20 December 2016 |
| Journal |
| Title of Journal |
The Journal of General and Applied Microbiology |
| Standard |
ISI |
| Institute of Journal |
Japan Science and Technology Information Aggregator, Electronic |
| ISBN/ISSN |
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| Volume |
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| Issue |
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| Month |
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| Year of Publication |
2017 |
| Page |
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| Abstract |
The use of probiotic bacteria to control bacterial infection in farmed fish is of clear practical interest. The aims of this study were to isolate and select a probiotic Bacillus sp. and to evaluate the effects of its supplementation on the growth and disease resistance of hybrid catfish. Bacillus siamensis B44v selectively isolated from Thai pickled vegetables (Phak-dong) displayed the high potential probiotic in catfish culture. This bacterium produced a bacteriocin-like substance and exhibited a broad-spectrum antibacterial activity inhibiting both Gram-positive and Gram-negative bacteria especially the fish pathogens Aeromonas hydrophila and Streptococcus agalactiae. The susceptibility to all 14 antibiotics tested implies its less possibility to be the antibiotic-resistant bacterium. Bacillus B44v possessed interesting adhesion properties as shown by its high percentages of hydrophobicity (64.8%), auto-agglutination (73.8%), co-aggregation (67.2% with A. hydrophila FW52 and 63.5% with S. agalactiae F3S) and mucin binding (88.7%). The strain B44v survived simulated gastrointestinal conditions and produced protease and cellulase enzymes. Hybrid catfish (C. macrocephalus x C. gariepinus) were employed in the feed-trial experiments. Fish fed diet containing strain B44v (107 CFU/g feed) displayed not only no mortality but also growth improvement. At the end of feed trial, fish were challenged by intraperitoneal injection of Aeromonas hydrophila FW52. The Bacillus B44v-fed fish survived (75.0%; p 0.05) better than the controls (36.7%; p 0.05) after a two week-challenge. These collective results present for the first time the potential of Bacillus siamensis B44v to be used as a bacterial probiotic in aquaculture. |
| Keyword |
aquaculture; Bacillus siamensis; catfish; fermented food; probiotic; Thai pickled vegetables |
| Author |
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| Reviewing Status |
มีผู้ประเมินอิสระ |
| Status |
ได้รับการตอบรับให้ตีพิมพ์ |
| Level of Publication |
นานาชาติ |
| citation |
true |
| Part of thesis |
true |
| Attach file |
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| Citation |
0
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Publication
Journal Publication
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