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Publication
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| Research Title |
Gene expression profiles during the transient ceftazidime resistance of Burkholderia pseudomallei |
| Date of Distribution |
10 June 2017 |
| Conference |
| Title of the Conference |
การประชุมวิชาการ โครงการปริญญาเอกกาญจนาภิเษก ครั้งที่ 18 (RGJ-Ph.D. Congress 18) |
| Organiser |
สกว |
| Conference Place |
Richmond Stylish Convention Hotel |
| Province/State |
Nonthaburi, Thailand |
| Conference Date |
8 June 2017 |
| To |
10 June 2017 |
| Proceeding Paper |
| Volume |
2017 |
| Issue |
1 |
| Page |
OWOH-P22 |
| Editors/edition/publisher |
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| Abstract |
Introduction and Objective
Burkholderia pseudomallei is a Gram-negative soil-dwelling bacillus and the etiological agent of melioidosis. The disease is the second most common cause of death among community-acquired bacteremia in the northeast Thailand. B. pseudomallei, is intrinsically resistant to several antibiotics but only a few were reported to resist against ceftazidime, the drug of choice. However, when the ceftazidime sensitive bacterium was grown in a biofilm-induced condition, it showed a transient resistant to the drug. Recently, the report of transient phenotypic changes, were referred to as the survival in response to lethal drug exposure. This study aimed to investigate the adaptive of bacterial metabolic shift during transient resistant state to shed some light to explain the drug transient resistant phenomenon of B. pseudomallei.
Methods
To demonstrate the gene expression profile during the bacterial transient antibiotic resistant, B. pseudomallei strain K96243 was grown as planktonic shaking, biofilm-induced and planktonic shedding cells and evaluated ceftazidime (CAZ) susceptibility by measuring the minimum inhibitory concentration (MIC). In addition, the gene expression profiles under these conditions were observed using the complete transcriptome profiles of RNA-sequencing in illumina processing platform. A set of genes with 3-folds changes of either higher or lower were quantitated the expression by quantitative reverse transcriptase PCR (qRT-PCR).
Results
By normalization using gene expression profile during planktonic shaking and comparing between biofilm and planktonic shedding cells, a set of 651 genes (29.8%) was differentially expressed higher or lower than the average. From the analysis, most of genes were expressed lower including energy metabolism and cell wall division that may reflect their response to environmental changes during biofilm-induced mode. On the other side, another group of genes involved nitrate respiration was expressed higher.
Conclusion
These results indicated the enhanced in variety of gene expression for adaptive metabolic shift in a mixture of biofilm population together with transient antibiotic resistant stage. Further investigation is required to prove if this metabolic shift plays any role in treatment failure and may lead to understand how bacteria survived in human host while the drug of choice is being used.
Key Words: Melioidosis, biofilm, antibiotic resistance, transient resistance, planktonic
References:
1. Sawasdidoln, C.; Taweechaisupapong, S.; Sermswan, R. W.; Tattawasart, U.; Tungpradabkul, S.; Wongratanacheewin, S. PLOS ONE 2010, 5 (2), e9196.
2. Hamad, M. A.; Austin, C. R.; Stewart, A. L.; Higgins, M.; Vazquez-Torres, A.; Voskuil, M. I. Antimicrobial agents and chemotherapy 2011, 55 (7), 3313-23.
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| Peer Review Status |
ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
ชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Poster |
| Part of thesis |
true |
| ใช้สำหรับสำเร็จการศึกษา |
ไม่เป็น |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
ไทย