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Publication
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| Research Title |
Reducing arsenic toxicity and accumulation in hydroponically grown rice seedlings with cadmium-tolerant and arsenite-oxidizing bacteria |
| Date of Distribution |
28 June 2017 |
| Conference |
| Title of the Conference |
2017 International Forum-Agriculture, Biology, and Life Science |
| Organiser |
University of Maryland, USA and Waseda University, Japan |
| Conference Place |
Kyoto International House |
| Province/State |
Kyoto, Japan |
| Conference Date |
27 June 2017 |
| To |
29 June 2017 |
| Proceeding Paper |
| Volume |
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| Issue |
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| Page |
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| Editors/edition/publisher |
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| Abstract |
Reducing arsenic toxicity and accumulation in hydroponically grown rice seedlings with cadmium-tolerant and arsenite-oxidizing bacteria
Sarun Thongnoka, Wilailak Siripornadulsila and Surasak Siripornadusila,b,c*
aDepartment of microbiology, Faculty of science, Khon Kaen University, Khon Kaen, Thailand
bResearch Center for Environmental and Hazardous Substance Management, Khon Kaen University, Khon Kaen, Thailand
cGenomics and Proteomics Research Group for Improvement of Salt-tolerant Rice, Khon Kaen University, Khon Kaen, Thailand
* Corresponding Author: surasak@kku.ac.th
ABSTRCT
Arsenic (As) contamination in environment is a serious problem worldwide in soil, water and crops affecting economy and risk to human health. Rice is a major crop with As accumulation potent due to irrigation and cultivation. In this study, the effect of cadmium (Cd)-tolerant and arsenite (As(III))-oxidizing bacteria on the reduction of As toxicity and accumulation in RD10 rice seedlings was demonstrated. Co-culture of Cd-tolerant Cupriavidus taiwanensis KKU2500-3 and As-oxidizing strains (4.25, 4.27, or 4.44) were able to colonize rice seedlings and grown in nutrient solution supplemented with 15 µM of As(III) and 30 µM of arsenate As(V). Compared with the un-inoculated control, bacteria enhanced rice growth parameters when grown in As solution. Shoot and root length and biomass increased by 1.42-38.36%. Co-inoculation with KKU2500-3 and 4.25 exhibited the highest shoot length and pigment content. While, co-inoculation with KKU2500-3 and 4.44 resulted in the highest root length and biomass. Rice seedlings inoculated with bacteria increased inorganic sulfide in roots and showed a negative correlation with As content in shoots (r=-0.97*). The arsenic content in shoot inoculated with KKU2500-3/4.25 was 41.84 mg/kg or reduced by 24.55% (translocation factor= 0.099) when compared with the control (55.45 mg/kg). However, the As content of these seedlings was higher than other co-inoculation (464.12 mg/kg). Rice seedlings inoculated with KKU2500-3 and 4.44 strains showed the lowest As accumulation at 354.56 mg/kg. The results indicated that co-inoculation of Cd-tolerant and As-oxidizing bacteria affect the ability to mitigate As toxicity and accumulation in rice seedlings.
Keywords: Cd-tolerant bacteria, As(III)-oxidizing bacteria, As accumulation, Arsenite, Arsenate
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ไม่มีผู้ประเมินอิสระ |
| Level of Conference |
นานาชาติ |
| Type of Proceeding |
Abstract |
| Type of Presentation |
Oral |
| Part of thesis |
true |
| Presentation awarding |
false |
| Attach file |
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| Citation |
0
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Journal Publication
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